Schematics of the bead motility assay. Red cones symbolize a focused trapping laser beam. (f) Example distance vs. time curves for CENP-E-coated beads moving on Taxol-stabilized microtubules; some beads coated with full-length CENP-E showed diffusive motions, similar to behavior of bead-free ...
After washed at least three times with the lysis buffer, the agarose beads were resuspended in sodium dodecyl sulfate (SDS) sample buffer (Nacalai Tesque, 09499-14) and heated for 5 min at 95 °C. The supernatant was collected after centrifugation at 1000 g for 2 min. Immuno...
The conversion of highly-soluble tau into its seed-competent, aggregation prone form involves the short hydrophobic domains of the MTBR normally responsible for binding to microtubules [48]. New structural information has also emerged that has given further insight into the mechanism of pathological ta...
工艺品设计 Prompt: Craft vector designs for a [specific type of craft item e.g., 'handmade necklace'] using materials like [specific materials e.g., 'beads, leather, and metal charms'], inspired by [specific theme or style e.g., 'vintage elegance']. Ensure it reflects the essence of...
The beads are magnetically captured, electrically stimulated and the light emission is proportional to the binding of ligand. A common label is Ruthenium (II) tri-bipyridine, NHS ester. electrodynamic forces London-Van der Waals forces: see DLVO theory. electrofocusing Any technique whereby chemical...
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Model filled polymers.Ⅷ.Synthesis of crosslinked polymeric beads by seed polymerization[J].Journal of Polymer Science Part A:Polymer Chemistry,1992.1463-1475.Zou, D., Sun, L., Aklonis, J.J., and Salovey, R., J. Polym. Sci., Part A: Polym. Chem. , 1992, vol. 30, p. 1463. ...
A small amount of 1 mm glass beads can be added and the mixture sonicated 3 times for about 5 seconds each time with a microprobe sonicator. The mixture is centrifuged and the protein concentration of the supernatant determined. One pg of protein from the soluble fraction of the culture can...
The canonical structure and function of centrioles are essential throughout mammalian development and physiology to form the cells’ cilia57. Centrioles serve as both the seed and template for cilia, and therefore, their precise structure is critical for their function57,58. Considering this major...