and left side of integument of 56 DAP fertile ovule; a-c represent the whole, chalazal end of 56 DAP abortive ovule, and left side of the bead quilt, respectively. D-G show the chalazal end of the fertile ovule 63 DAP, spherical embryo and its cells, right side of the integument,...
hiPSC-derived ECs (hiPSC-ECs) that underwent CD144 antibody-conjugated magnetic bead sorting exhibited typical EC morphological and functional characteristics, including tubular structure formation, responsiveness to inflammatory signals, and nitric oxide production [23]. Notably, the hiPSC-ECs demonstrated ...
After washing six times with bead-binding buffer without BSA or ethidium bromide, but containing the protease inhibitors set, the bound proteins were eluted with 2 Â SDS–polyacrylamide gel electrophoresis (PAGE) loading buffer and analysed by 12% SDS–PAGE. After electrophoresis, the gels were...
™ ™ ®Coupled with this has been the development of diverse methods for SNP genotyping, such as Illumina's BeadArray technology based GoldenGate and Infinium assays, Life Technologies TaqMan assay and KBiosciences Competitive Allele Specific PCR (KASPar )11. In addition to these...
Arrows indicate regions where AgNPs (dark bead like structures) have accumulated in treated seeds. Full size image Effect of nano-treatments on total phenolics, radical scavenging activity, and elemental composition in watermelon fruit Phenolic compounds have high antioxidant activities and free radical ...
Peptide samples were loaded onto an Acclaim PepMap RSLC reversed-phase column (75 μm inner diameter, 25 cm length, 2 µm bead size (Thermo Scientific, Waltham, MA, USA) at a flow rate of 0.4 μL min−1 in 3% (v/v) acetonitrile, 0.1% (v/v) formic acid. Peptides were eluted...
Magnetic bead-based colorimetric immunoassay for aflatoxin B1 using gold nanoparticles. Sensors 2014, 14, 21535–21548. [Google Scholar] [CrossRef] [Green Version] Mohammed, A.M. Fabrication and characterization of gold nano particles for DNA biosensor applications. Chin. Chem. Lett. 2016, 27, ...
The adipokine bead panel, which includes IL-6, MCP-1, TNF-α, PAI-1, insulin, leptin, and resistin, was quantified using the commercial kit MAD-KMAG-71K (Merck-Sigma Aldrich, São Paulo, Brazil). The plates were analyzed on a Luminex MAGPIX System (Luminex Corporation, Austin, TX, ...
(cells/bead ratio), and the agitation speed were varied based on predefined experimental runs inSection 2.10.2. During the entire culture period, the minimum dissolved oxygen (DO) was set at 50% saturated air and controlled by the addition of air enriched with a maximum of 40% oxygen ...
Upon using PS in PDMS as a carrier polymer, well-defined bead-free fibers were obtained by a direct electrospinning solution containing PS/PDMS/THF (1:1:10). Plasma treatment acted as an adhesion promoter between the polymeric substrate and PS/PDMS electrospun fiber mats, which was confirmed ...