Dual RNA-seq转录组相互作用利用转录组测序技术(RNA-sequencing,RNA-seq)进行转录组分析是了解病原体入侵宿主分子变化的重要工具,在同时分析病原体与宿主转录组时,RNA-seq技术需要分别构建病原体及宿主的cDNA文库,再将其各自映射到病原体及宿主参考基因组中,而互作转录组测序技术(Dual RNA-seq)无需分离两物种,只...
互作转录组测序(DualRNA-seq) 1 互作转录组测序(DualRNA-seq) 物种间存在广泛的相互作用,包括寄生、共生、竞争等,而普通的转录组只能研究单一物种的信息,不 仅浪费一部分数据,而且在分离中也会对样本本身造成一定的影响。 互作转录组(DualRNA-seq),无需分离两物种,避免分离造成的干扰,只构建一个转录组文库,便能...
作者在其Dual RNA-seq数据中,发现有稳定的rRNA残留,且残留都是来源于23S固定的区域(下图上),因此开始了测试: 1.作者首先验证Dual RNA-seq数据中残留的部分rRNA是否可以表征Total rRNA的量,通过qPCR和测序数据的对比,作者发现残留的rRNA和真实...
Technological developments in DNA sequencing and bioinformatics have laid the foundation for dual RNA sequencing (dual RNA-seq) with which the responses of a pathogen and host can be defined simultaneously13,14,15. This is valuable because analysis of the host and the pathogen concurrently using a...
DualRNA-seq高通量测序数据分析软件是由武汉康测科技有限公司著作的软件著作,该软件著作登记号为:2023SR1435929,属于分类,想要查询更多关于DualRNA-seq高通量测序数据分析软件著作的著作权信息就到天眼查官网!
seq转录rnadual物种宿主 互作转录组测序(DualRNA-seq) 1 互作转录组测序(DualRNA-seq) 物种间存在广泛的相互作用,包括寄生、共生、竞争等,而普通的转录组只能研究单一物种的信息,不 仅浪费一部分数据,而且在分离中也会对样本本身造成一定的影响。 互作转录组(DualRNA-seq),无需分离两物种,避免分离造成的干扰,只...
(2012). A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity. Science 337, 816-821. Salvatore,Incontro,S Cedric,... - 《Neuron》 被引量: 121发表: 2014年 RNA-guided editing of bacterial genomes using CRISPR-Cas systems Here we use the clustered, regularly interspaced...
To elucidate howB. cinereatranscriptional reprogramming during infection ofA. thaliana, we performed dual RNA-seq analysis. Illumina sequencing was performed on mRNA libraries extracted from a mixture of whole plants and fungi at different time points post infection (0–96 hpi). Conidia at 0 h and...
To monitor gene regulation, coaggregates were formed in human saliva and, after 30minutes, whole-transcriptomes were extracted for sequencing and Dual RNA-Seq analysis. In total, 272 genes were regulated in, including 39 genes in oxidoreductase processes. In, there was a high degree of inter-...
Rai et al., 2014 Dual RNA-seq of the plant pathogen Phytophthora ramorum and its tanoak host. Tree Genet. Genomes 10(3): 489-502. https://doi.org/ 10.1007/s11295-014-0698-0Hayden KJ, Garbelotto M, Knaus BJ, Cronn RC, Rai H, Wright JW. Dual RNA- seq of the plant pathogen ...