Reporter expression was observed in nascent compartments in transgenic mouse embryos, and mESCs were used to develop differentiation assays in which chemical modulators of Wnt (XAV939: 3, 10M), retinoic acid (all-trans retinoic acid: 0.1, 1, 10M; 9-cis retinoic acid: 0.1, 1, 10M; ...
3c, d), underscoring the sensitivity and specificity of this dual reporter platform. Moreover, we tested isoprenaline, a drug that has been demonstrated to accelerate the heat beating [27], in the generated CMs from our reporter cell line. Consistent with previous observations, isoprenaline ...
The invention discloses a cell line of dengue virus type-2 replicor with a dual-reporter gene and an application. By constructing a dengue virus type-2 replicor with the dual-reporter gene and transfecting cells, a cell line of dengue virus type-2 replicor with the dual-reporter gene, CCTC...
NF-κB–SEAP and IRF–Lucia reporter HEK293 cells expressing human TLR9 Signaling pathways in HEK-Dual™ hTLR9 cells HEK-Dual™ hTLR9 cellswere were engineered fromHEK-Dual™ cells, a human embryonic kidney HEK293-derived cell line, to study the Toll-like receptor 9 (TLR9)-dependent NF...
NF-κB–SEAP and IRF–Lucia reporter HEK293 cells expressing human TLR7 Signaling pathways in HEK-Dual™ hTLR7 cells (click to enlarge and see legend) HEK-Dual™ hTLR7 cellswere engineered fromHEK-Dual™ cells, a human embryonic kidney HEK293-derived cell line, to study the human Toll...
MC38 (murine colon adenocarcinoma, Kerafast ENH204-FP) and Luciferase/tdTomato dual-reporter MC38 (murine colon adenocarcinoma, Alstem LRL04) (MC38-Fluc-RFP) cell lines were purchased from Kerafast or Alstem Cell Advancements, respectively. The MDA-MB-231-FLuc-GFP (human triple-negative breast...
TIGIT&PVRIG Dual Effector Reporter Cell 报告基因药靶模型很好的模拟了体内 TIGIT&PVRIG 的信号转导过程,原理见下图所示。 Figure 1.TIGIT&PVRIG Dual Effector Reporter Cell 细胞模型原理图 III. Introduction Expressed gene:TIGIT/PVRIG Stability:32 passages (in-house test, that not means the cell line wil...
Validation of a Reporter Cell Line for Flavivirus Inhibition Assays Here, we report the validation of a new reporter cell line, Hec1a-IFNB-Luc, for use in inhibition studies of various flaviviruses relevant to human patholo... Tatiana M. T. Rezende,Gabriella Macera,Leo Heyndrickx,... - ...
The finding that Dox is required for all reporter gene expression demonstrates that reporter production is under the complete control of the tight-TRE promoter (Supplementary Fig. 2). We then introduced the NIrD system to a hepatic cell line, Huh7.5, and selected Blasticidin-resistant clones for...
报告基因细胞系 REPORTER CELL LINES 现代细胞生物学研究,对靶标基因的示踪与表达标记已成为不可或缺的研究手段之一。美森细胞在长期的积累中,开发了独具特色的高效率基因敲入技术和可实现单细胞分辨率的体内示踪标记方法。针对客户所需要的靶标基因,可实现高达20%的大片段报告基因靶向高效率敲入。依靠快速稳定的转基因...