Reducing agent Cleaves disulfide bonds Supplier Page Compare Product DTT (Dithiothrietol) Quantity: 25g Applications: Reducing agent Cleaves disulfide bonds Citations: (1) Supplier Page Compare Product OneQuant™ DTT [0.5M] Quantity: 40 x 7.7mg ...
只是知道点皮毛而已,就我了解的而言,western blot要得以进行,特别是抗体要和目的蛋白结合,一方面要使打开蛋白质的SS键,因为如果SS键不打开,很有可能抗体不能很好的识别特定的位点,就不能结合上去;另一方面还要用SDS-PAGE消除蛋白质分子内的各种非共价键,使蛋白质所带电荷和其分子量成正比,这样才能使蛋白质分离出来。
DTT is a strong reducing agent, and its reducing properties are largely due to the conformational stability of its six-membered ring (containing disulfide bonds) in its oxidized state. Its redox potential is -0.33 volts at pH 7. The reduction of a typical disulfide bond by DL-Dithiothreitol c...
DTT is an unusually strong reducing agent, owing to its high conformational propensity to form a six-membered ring with an internal disulfide bond. It has a redox potential of -0.33 V at pH 7. The reduction of a typical disulfide bond proceeds by two sequential thiol-disulfide exchange reacti...
We detected TGF-β monomers with the apparent molecular weight of 12.5 kDa in samples treated alone with either NAC or DTT by Western blot analysis (Figure 3B) suggesting that these reducing agents promoted the formation of TGF-β monomers. Notably, addition of diamide restored detection of the...
The lysate was mixed with 4 × NuPAGE® LDS sample buffer and 10 × sample reducing agent, and proteins were denaturated at 100℃ for 10 min. NuPAGE® Novex® 4–12% Bis-Tris Protein Gels (Thermo Fisher Scientific) were used for protein electrophoresis under a 100 V...
只是知道点皮毛而已,就我了解的而言,western blot要得以进行,特别是抗体要和目的蛋白结合,一方面要使打开蛋白质的SS键,因为如果SS键不打开,很有可能抗体不能很好的识别特定的位点,就不能结合上去;另一方面还要用SDS-PAGE消除蛋白质分子内的各种非共价键,使蛋白质所带电荷和其分子量成正比,这样才能使蛋白质分离出来...