coli HT115 (DE3) for the production of dsRNA. Notably, the variable that had the greatest effect on dsRNA yield was cultivation in TB medium, which resulted in a 118% increase in yield. Furthermore, lactose induction (6 g/L) yielded 10 times more than IPTG. Additionally, our optimized ...
可通过在器官、组织或细胞类型中的特异性转录;环境条件(例如,感染、胁迫、温度、化学诱导物)的刺激;和/或在发育期或年龄的工程转录(engineeringtranscription)来粑向抑制。RNA链可被聚腺苷酸化或者可不,皮聚腺苷酸化;RNA链可以能够通过细胞的翻译设备翻译为多肽,或者可以不能这样。的RNA、dsRNA、siRNA或miRNA,或在...
Ziebell H, MacDiarmid R (2017) Prospects for engineering and improvement of cross-protective virus strains. Curr Opin Virol 26:8–14 Article PubMed Google Scholar Zotti M, Dos Santos EA, Cagliari D, Christiaens O, Taning CNT, Smagghe G (2018) RNA interference technology in crop protection ...
最近在提取一种病毒（RNA病毒），它在复制过程中是以双链形式存在的，由于量较大所以想寻求一种简便的...
As a complement to traditional breeding, biotechnological engineering has been an alternative approach to producing virus-resistant crops. Abel et al. showed that transgenic tobacco plants expressing the coat protein (CP) gene of tobacco mosaic virus are resistant to the virus50. This method, referre...
Genetic Engineering & Biotechnology News. 2019 Dec 19; 39(12):56-58. [2] Schönborn J, Oberstrass J, Breyel E, Tittgen J, Schumacher J, Lukacs N. Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res. 1991 Jun 11;...
[1] Bijoyita Roy, Monica Z. Wu. Understanding and Overcoming the Immune Response from Synthetic mRNAs. Genetic Engineering & Biotechnology News. 2019 Dec 19; 39(12):56-58. [2] Schönborn J, Oberstrass J, Breyel E, Tittgen J, Schumacher J, Lukacs N. Monoclonal antibodies to double-...
dsRNA-mediated Gene Silence and Antiviral Genetic Engineering; 双链RNA激发的植物基因沉默及其在植物育种上的应用 2. dsRNAIsolation Method and Research of Plant Virology; 双链RNA技术与植物病毒研究 3. In order to produce a marked effect in RNAi,21-25 nt siRNA,which is fromdsRNAcutted by nucleinas...
Biotechnology and Bioprocess Engineering, 2007, 12: 318-322. [2] 胡同乐, 曹克强, 王树桐, 甄文超. 生长季苹果斑点落叶病流行主 导因素的确定. 植物病理学报, 2005, 35(4): 374-377. HU T L, CAO K Q, WANG S T, ZHEN W C. Study on the main factor for the epidemic of Alternaria blotch...
Nanjing Drum Tower Hospital Center of Molecular Diagnostic and Therapy, State Key Laboratory of Pharmaceutical Biotechnology, Jiangsu Engineering Research Center for MicroRNA Biology and Biotechnology, NJU Advanced Institute of Life Sciences (NAILS), School of Life Sciences, Nanjing University, Nanjing, Ji...