培训内容:高通量全自动快速DSF 蛋白质稳定性分析技术原理及应用培训时间:2024年9月10日(周二)14:00-15:00培训地点:清华大学生物医学馆E203会议室培训方式:线上线下结合报告人:Tim Flanagan英国Protein Stable 公司联系方式:常老师changing#tsing...
品牌:Protein Stable 生产商:Applied Photophysics 应用光物理 所在地:国外 产地:英国 仪器原理:差示扫描荧光法 (DSF,内源荧光法) 产品简介:差示扫描荧光法 (DSF,内源荧光法)是一种非标记的生物物理技术,其原理是通过测量温度升高时或在变性剂存在下tryptophan ...
Method: Differential scanning fluorimetry assay (DSF)DSF experiments were performed using Bio-Rad CFX ConnectTM Real-Time System. First, 9.8 μL protein and SYPRO Orange mixture were added per well, and then mixed 0.2 μL compound. The final assay volume is 10 μL. The final assay...
SUPR-DSF 来自于Protein Stable。 Protein Stable是英国应用光物理(Applied Photophysics-APP)和Fluorescence Innovations(FI)的合资企业。Protein Stable 致力于将蛋白表征方面的创新技术用于帮助学术界和生物技术行业的科学家、研究人员。 聚焦于高通量、低样品量的蛋白表征方法,以帮助用户提高生产率,数据质量。 >>> 关于...
DSF experiments were performed using Bio-Rad CFX ConnectTM Real-Time System. First, 9.8 μL protein and SYPRO Orange mixture were added per well, and then mixed 0.2 μL compound. The final assay volume is 10 μL. The final assay system contained 20 mM Hepes, pH 7.5 150 ...
This suggests that it should be possible, in theory, to fold any protein. In practice, however, protein production by refolding is challenging because suitable refolding conditions must be empirically determined for each protein and can be painstaking. Here we demonstrate, using a variety of ...
Biter AB, de la Pena AH, Thapar R, Lin JZ, Phillips KJ (2016) DSF guided refolding as a novel method of protein production. Sci Rep 6:18906Biter A. B. et al. , DSF guided refolding as a novel method of protein production . Sci. Rep. 6 , 18906 (2016)....
Comparison of originator Fc-fusion protein with 16 biosimilar candidates 3. 酶稳定性高通量筛选 酶是一类极为重要的生物催化剂。对于工业用途来说,酶必须拥有足够的稳定性以适应不同的反应条件,如高浓度底物,表面活性剂的添加,非常规的 pH 和温度。我们可以利用酶的热力学稳定性对其评估,从酶库中筛选出理想的...
Senior Scientist of Protein Biophysics 【浦东新区】 面议 1-3年博士五险一金年终奖金绩效奖金餐费补贴 药明康德 制药,生物技术,医药外包不需要融资10000人以上 王女士 Recruiting Consultant 结构生物学组长(结晶,x-ray) 【苏州】 20-40k·14薪 经验不限博士 ...
When a thermal or chemical denaturant gradient is applied to a protein sample, it will begin to unfold under thermal or chemical duress. Researchers use the point at which unfolding occurs as an indication of the overall impact a modification or condition has on stability. Changes to the ...