Comparison of originator Fc-fusion protein with 16 biosimilar candidates 3. 酶稳定性高通量筛选 酶是一类极为重要的生物催化剂。对于工业用途来说,酶必须拥有足够的稳定性以适应不同的反应条件,如高浓度底物,表面活性剂的添加,非常规的 pH 和温度。我们可以利用酶的热力学稳定性对其评估,从酶库中筛选出理想的...
Quickly characterize protein stability for construct and formulation screeningTech Aunty Speed-up stability studies With differential scanning fluorimetry (DSF), you can quickly screen through many different proteins or formulation candidates to find the most stable ones. Instead of waiting days or weeks...
protein melting temperatureDifferential scanning fluorimetry (DSF) is a widely used thermal shift assay for measuring protein stability and protein-ligand interactions that are simple, cheap, and amenable to high throughput. However, data analysis remains a challenge, requiring improved methods. Here, ...
When it comes to protein stability analysis in biopharmaceutical research, Differential Scanning Calorimetry (DSC) offers unmatched accuracy and versatility com
此外，药物亲和反应靶点稳定技术 (drug affinity responsive target stability, DARTS) 检测表明，BZ 可减少蛋白水解 (图 4G)，这证实了 BZ 与 DOHH 的相互作用。综上结果：DOHH 是 BZ 的直接细胞靶蛋白。研究人员发现代表着向开发具有神经保护作用的 DOHH 小分子异位激活剂迈出了关键的第一步。该研究还证明 DOHH...
细胞热迁移技术 (CETSA) 也显示,与对照组相比,BZ 增加了 DOHH 的热稳定性 (图 4F)。此外,药物亲和反应靶点稳定技术 (drug affinity responsive target stability, DARTS) 检测表明,BZ 可减少蛋白水解 (图 4G),这证实了 BZ 与 DOHH 的相互作用。综上结果:DOHH 是 BZ 的直接细胞靶蛋白。
Comparison of originator Fc-fusion protein with 16 biosimilar candidates 3.酶库高通量筛选 酶是一类极为重要的生物催化剂。对于工业用途来说,酶必须拥有足够的稳定性以适应不同的反应条件,如高浓度底物,表面活性剂的添加,非常规的pH和温度。我们可以利用酶的热力学稳定性对其评估,从酶库中筛选出理想的候选物。
Unveil protein stability & ligand binding with 2bind's nanoDSF expertise. High-throughput screening, quality control, formulation development to empower your biologics & drug discovery.
Nemeth. "The Effects of Intramolecular and Intermolecular Electrostatic Repulsions on the Stability and Aggregation of NISTmAb Revealed by HDX-MS, DSC and NanoDSF". Protein Science. Vol. 30, Issue 8, p. 1686-1700. 2021. Protein Stable 21 Mole Business Park Leatherhead Surry KT22 7BA UK ...
细胞热迁移技术 (CETSA) 也显示,与对照组相比,BZ 增加了 DOHH 的热稳定性 (图 4F)。此外,药物亲和反应靶点稳定技术 (drug affinity responsive target stability, DARTS) 检测表明,BZ 可减少蛋白水解 (图 4G),这证实了 BZ 与 DOHH 的相互作用。综上结果:DOHH 是 BZ 的直接细胞靶蛋白。