To evaluate the role of serum IgG, IgM and IgA anti-dsDNA antibody isotypes in the diagnosis of systemic lupus erythematosus (SLE), and their association with clinical features and disease activity, in a large cohort of SLE patients.Sera of 200 SLE patients (mean age 34±10.3 years; 26 ...
10. The chimeric antibody of claim 1, wherein the chimeric antibody specifically binds human dsDNA and chromatin. 11. (canceled) 12. The chimeric antibody of claim 1, wherein the chimeric antibody has a linear dilution profile within a range of 10-9000 relative fluorescence intensity (RFI)...
False-positive test results can be seen when impure DNA is used as a substrate. The qualitative properties of anti-dsDNA, including antibody avidity, complement fixing property, and Ig class, may affect their pathogenicity. The various clinical tests for anti-dsDNA preferentially measure antibodies ...
Of the 218 AAV patients, 109 (50.0%) were positive for ANA, 45 (20.6%) were positive for anti-dsDNA, and 43 (19.7%) were positive for both. The AAV patients with ANA had severer kidney damage and more chronic renal histopathological changes compared to those who were negative for ANA....
Mouse monoclonal anti-H3 antibody Active Motif Cat# 61475; RRID: AB_2687473 Rabbit polyclonal anti-H2A.Z antibody Active Motif Cat# 39647; RRID: AB_2793289 Mouse monoclonal anti-Myc antibody Millipore Cat# 05–724; RRID: AB_11211891 Chemicals, peptides, and recombinant proteins Dynabeads Protein...
Here, we performed 147,016 Anti-dsDNA antibody measurements by Farr-RIPA, a precise method with a broad analytic range as compared to other methods which have a limited upper limit of quantitation or are semi-quantitative. Our 7800 positive Farr-RIPA results exhibited quantitative results from ...
Kim KH, Han JY, Kim JM et al (2007) Clinical significance of ELISA positive and immunofluorescence negative anti-dsDNA antibody. Clin Chim Acta 380:182-185Kim KH, Han JY, Kim JM, Lee SW, Chung WT. Clinical signif- icance of ELISA positive and immunofluorescence negative anti-dsDNA ...
Anti-dsDNA antibody levels in SLE patient specimens (N = 105) were measured and assessed for clinical performance using manufacturer cut-off limits along with the Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K) score. Results Assay precision, measurable range and normal ...
Results A 56-year-old female patient, diagnosed with systemic lupus erythematosus since 2015 characterized by typical malar erythema, photosensitivity, polyarthritis, leucopenia, positive antinuclear antibody, anti-dsDNA, and anti-chromatin antibody. She received hydroxychloroquine and prednisone. After 1 ...