例子是疱疹病毒的TK启动子、SV40早期启动子、酵母gal4基因启动子等。可以选择转录起始调控信号,使其具有阻遏和激活作用,从而能够调节基因的表达。 将包含编码本发明蛋白的核苷酸序列的DNA分子插入载体中与转录和翻译调控信号操作性相连,该载体能将所需基因序列整合入宿主细胞中。 还可以通过导入一个或多个允许选择含有表...
b Autophagy flux visualized using GFP-mCherry-Atg8a in midgut cells from control (NP1-GAL4/UAS-GFP-mCherry-Atg8a), Atg1RNAi (NP1-GAL4/UAS-GFP-mCherry-Atg8a; UAS-Atg1RNAi/+), and Dpp (NP1-GAL4/UAS-GFP-mCherry-Atg8a; UAS-dpp/+) larvae at −4 h RPF. Scale bar ...
As insc-GAL4 mediated Rab11RNAi in Drosophila larval and adult midguts show the disruption of the niche microenvironment of adult midgut precursors as well as elevated DPP signalling at the larval stage, which is associated with aberrant over-proliferation and early differentiation of larval AMPs ...
2A); dppblk-Gal4 that is expressed in the eye imaginal margins (Fig. 2F); and GMR-Gal4 that is expressed posterior to the MF (Fig. 2K). The expression of punt RNAi driven by optix-Gal4 driver decreased MF progression and retinal differentiation (Fig. 2B-C, P), and both the co-...
c587Gal4 is strongly expressed in CySCs and somatic cyst cells of the Drosophila testis (Fig. 1a). Our recent data show that Tkv acts as receptor trap to restrain Dpp signaling within the niche53. Surprisingly, we found that when a constitutively active form of tkv (tkvCA) was expressed...
Dpp was overexpressed in either all CFCs in C532-GAL4, UAS-dpp females (A–H) or FLP-out clones of follicle cells in egg chambers from hsp70-flp; tub>polyA>GAL4, UAS-lacZ/UAS-dpp females (I–N). In all micrographs, anterior is to the left. (A–C) Dpp overexpressing stage 10B ...
To determine the function of Notch signaling in interstitial cells, cell-type-specific GAL4 driver lines are required. Thus, we screened GAL4 enhancer-trap lines whose expression is detected in the first instar larval midgut, and identified several useful ones. (1) All midgut lines (NP3012all...
To this end, we used the tubulin-Gal4 (tub-Gal4) driver to mediate ubiquitous expression of upstream activating sequence (UAS)-RNAi’s targeting each of the transferases. Analysis of cuticle phenotypes revealed that loss of the super sex combs-encoded OGT, via a UAS-RNAi transgene targeting...
(A) Overexpression of TkvQD (UAS-TkvQD) by esg::Gal4ts in the CCR induces high Dpp signaling activity, shown by pMad antibody staining, compared to the control. Arrowheads point to esg+ cells. (B) Cells in Dad212 mutant MARCM clones (arrowhead) have higher Dpp signaling activity, shown...
(B–C”’) Hth+Tsh-expressing clones, marked by anti-HA, were induced in the eye and wing imaginal discs at 72–96 h after egg laying using the Gal4/UAS system; simultaneously in the same discs eGFP::Dpp was expressed using the dpp-lexA driver through the lexA/lexO system. Hth+Tsh...