Telomeres are bound by dedicated proteins, which protect them from DNA damage and regulate telomere length homeostasis. In the nematode Caenorhabditis elegans, a comprehensive understanding of the proteins interacting with the telomere sequence is lackin
We devised a facile method for mapping DNA–protein interaction sites in vivo using the double-stranded DNA-specific cytosine deaminase toxin DddA. In 3D-seq (DddA-sequencing), strains containing DddA fused to a DNA-binding protein of interest accumulate characteristic mutations in DNA sequence ...
Bidirectional replication in eukaryotes starts when the paths of two helicase rings cross as they translocate along single-stranded DNA, with their N-terminal domain forming the leading edge of the advancing replication machinery. Through this mechanism, the DNA strand excluded from the central pore o...
The ability of a cancer cell to repair double-stranded DNA breaks with a BRCA2 mutation is impaired; however, further repair of damage is possible through the activity of PARP. In May 2020, the FDA approved the oral PARP inhibitors rucaparib (Rubraca) and olaparib (Lynparza) for the ...