QingdaoTaoQingdaoZhaoQingdaoHaijieQingdaoLiuQingdaoQiQingdaoShiQingdaoSensors and Actuators, B. ChemicalShi C, Liu Q, Zhou M, Zhao H, Yang T, Ma C. Nicking endonuclease-mediated isothermal exponential amplification for double-stranded DNA detection. Sens Actuators B Chem. 2016; 222: 221-5....
Double-stranded DNA (dsDNA) has been used for CRISPR homology directed repair (HDR) template for creating gene knock-in with high editing efficiency. GenWand™ dsDNA is developed with covalently closed ends to mitigate no-homologous end joining risk and increase knock-in accuracy. GenScript now...
High Avidity Double-Stranded DNA (HA dsDNA) Antibody, IgG FOR THE DETECTION OF HIGH-AVIDITY AUTOANTIBODIES AGAINST DOUBLE-STRANDED DNA (DSDNA) ARUP LABORATORIES | MAY 2012 2005526 High Avidity Double-Stranded DNA (HA dsDNA) Antibody, IgG For speci c collection, transport, and testing information,...
Details of the method are provided in protocol entitled Analysis of double-stranded DNA fragments using capillary electrophoresis.1Briefly, TOTO™-1, an inexpensive fluorescent DNA intercalating dye, is incubated with a DNA sample for approximately 10 minutes in water. The sample is then ...
single-stranded and double-stranded DNA binding proteins based on protein sequences Wei Wang1,2*, Lin Sun1, Shiguang Zhang1, Hongjun Zhang3, Jinling Shi4, Tianhe Xu1 and Keliang Li1 Abstract Background: DNA-binding proteins perform important functions in a great number of biological activities...
Precise genome editing involves homologous recombination between donor DNA and chromosomal sequences subjected to double-stranded DNA breaks made by programmable nucleases. Ideally, genome editing should be efficient, specific, and accurate. However, bes
Evaluation of global and double-stranded sperm DNA fragmentation: alkaline and neutral Comet assays The Comet assay was used to determine sperm DNA damage in each of the 25 biological replicates. The incidence of double-stranded ...
SMART-SLE: serology monitoring and repeat testing in systemic lupus erythematosus—an analysis of anti-double-stranded DNA monitoring systemic lupus erythematosustest monitoringOBJECTIVE. Disease activity monitoring in SLE includes serial measurement of anti-double stranded-DNA (dsDNA) antibodies... YA Li...
an increasing number of DNA damage-repair-related genes have been linked with the occurrence of POI. Among them, DNA double-strand breaks (DSBs), one of the most damaging to DNA, and its main repair methods including homologous recombination (HR) and non-homologous end joining (NHEJ) are of...
Single-stranded cDNA was converted to dsDNA by DNA polymerase I while incorporating dU/VTPs (10 mM). Samples were fragmented in 1 × TE to 200–300 bp using Covaris. After fragmentation, samples were purified using the MinElute PCR purification kit (Qiagen). Fragmented samples underwent ...