This value can best be estimated from cell loading densities into the 10X/Drop-Seq device, and adjusted according to the estimated proportion of homotypic doublets. Step2: 确定参数,寻找最优pK值 对Cell哈希和Demuxlet数据集进行pN- pk参数扫描的ROC分析表明,DoubletFinder的性能在很大程度上不受pN值...
T cell differentiation requires Notch1 signaling. In the present study, we show that an enhancer upstream of Notch1 active in double-negative (DN) mouse thymocytes is responsible for raising Notch1 signaling intrathymically. This enhancer is required to expand multipotent progenitors intrathymically wh...
Cell Death Differ. 13: 1213-1221.C. Verschelde, D. Michonneau, M.C. Trescol-Biemont, I. Berberich, A. Schimpl, N. Bonnefoy-Berard, Overexpression of the antiapoptotic protein A1 promotes the survival of double positive thymocytes awaiting positive selection, Cell Death Differ. 13 (2006) ...
Double Positive Thymocytes Select Mucosal-Associated Invariant T Cells. During thymic development, NKTs are selected by CD1dexpressing cortical thymocytes; however, the hematopoietic cell type responsible for MAIT cell selection ... Seach,Natalie,Guerri,... - 《Journal of Immunology》 被引量: 73发表...
During thymic development, NKTs are selected by CD1dexpressing cortical thymocytes; however, the hematopoietic cell type responsible for MAIT cell selection remains unresolved. Using reaggregated thymic organ culture and bone marrow chimeras, we demonstrate that positive selection of mouse iV伪19 ...
Thymocytes displaying self-reactive T cell receptors usually undergo negative selection in the thymus. Here we demonstrate that agonist peptides can promote positive selection of immature double-positive thymocytes into distinct lineages, varying with th
We simulated seven doublet-containing scRNA-seq datasets with pre-defined cell types, DE genes, and cell trajectories. Then, we evaluated the accuracy of the four downstream analyses by their state-of-the-art computational methods before and after doublets were removed by each doublet-detection ...
The bclx gene has been shown to regulate programmed cell death in vitro. We now show that Bclx expression increases dramatically when T cells differentiate from CD4- CD8- (double negative) thymocytes to CD4+ CD8+ [double positive (DP)] thymocytes. In contrast single-positive (SP) thymocytes...
During T cell development in the thymus, pre–T cell receptor (TCR) complexes signal CD4CD8(double negative [DN]) thymocytes to differentiate into CD4+CD8+(double positive [DP]) thymocytes, and they generate such signals without apparent ligand engagements. Although ligand-independent signaling is...
Acute thymic atrophy has also been observed in sepsis and is characterized by the specific loss of CD4+CD8+ double-positive thymocytes, thus resulting in a dramatic reduction in T-cell development and immunosuppression12,13. It is well known that thymic atrophy is caused by microbial infection-...