The weaker of the (Ca2+)4-CaM-binding sites was known via electrophysiology to have a role in long-term inactivation of the channel but not known to bind CaM. FGF12A binding to a complex of CaM associated with a fragment of the NaV1.2 CTD increased the Ca2+-binding affinity of both ...
Despite these differences, we observed that both DBD and TAD mutant forms of p53 interact with the EGFR. The EGFR-AKT-PI3K-mTOR axis plays a central role in cellular growth and metabolism and is frequently altered in cancer. We therefore investigated whether TAD versus DBD forms of mutant p53...
However, in contrast to its role in activation of the K10 promoter, this effect is independent of DNA binding. The POU domain, which is normally responsible for DNA binding, is the primary mediator of the repression. The repression maps to the proximal promoter region of the K14 gene. This...
To investigate the functions of the GnT-IVa lectin domain in more detail, we purified soluble wild-type enzyme and its D445A mutant (Fig.5a, b) and measured their enzymatic activity. The activity of D445A was reduced to ~62% of that of the wild type (Fig.5c), indicating that the g...
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The phosphatidylinositol-4, 5-bisphosphate 3-kinase (PI3K)-Akt signaling pathway plays an important role in breast cancer development. As the most critical signal molecule in this pathway, p-Akt has attracted considerable attention as a therapeutic target [11]. p-Akt is associated with resistance...
The role of autophagy in cancer onset and progression remains controversial as it may promote or hinder tumor progression depending on the tumor type and stage. In GBMs, autophagy has been often associated with chemoresistance mechanisms, although growing evidence has also indicated that autophagy indu...
(2020), working with nineteen potent neutralizing antibodies (in vitro) obtained from infected patients, found that almost half of them were directed against the RBD [10], highlighting its potential role as a vaccine antigen. For these reasons, SARS-CoV-2 RBD was selected for its heterologous ...
The ratios suggest purifying selection eliminated mutants in the kinase domain but not the LRR domain. The relative lack of amino acid sequence variance per nucleotide change may be associated with the role of the kinase domain in the signaling function. Appropriate message transduction imposes severe...
Although the physiological role of LytM and its catalytic domain remains uncertain, the catalytic domain has properties that could make it attractive as a potential antistaphylococcal agent. First, the protein can be easily overexpressed in Escherichia coli with very high yields and is easy to ...