Dof has only one conserved zinc finger domain containing 52 amino acids. In general,the zinc finger domain contains 4 conserved Cys residues and one Zn2+,and these two components are both indispensable for Dof protein. Cysteine mutation or the presence of a divalent ion chelating agent will ...
encoding a Dof zinc finger domain containing protein,was the candidate gene of LD1.Further analysis showed Amino acid cysteine(C) to arginine(R) mutation was likely to lead to the zinc finger protein deactivation.Point mutation analysis reveals that cysteine at position 51 was critical to LD1 ...
AOBP, a DNA-binding protein in pumpkin, contains a Dof domain that is composed of 52 amino acid residues and is highly conserved in several DNA-binding proteins of higher plants. The Dof domain has a significant resemblance to Cys2/Cys2 zinc finger DNA-binding domains of steroid hormone rec...
Dof转录因子在植物中的调控作用
(1998). Functional analyses of the Dof domain, a zinc finger DNA-binding domain, in a pumpkin DNA-binding protein AOBP. FEBS Lett. 430 , 251–256. [ PubMed ]Shimofurutani, N., Kisu, Y., Suzuki, M. and Esaka, M. (1998) Functional analyses of the Dof domain, a zinc fnger DNA...
The Dof domain, is a DNA-binding domain that contains a C2C2-type zinc finger motif that recognizes a cis-regulatory element with a common core sequence of 5′-(A/T)AAAG -3′, except a pumpkin Dof protein designated as AOBP that recognizes an 5′-AGTA- 3′ sequence4...
namely Dof domain which contains a single C2C2-type zinc-finger- like motif and specifically recognizes an (A/T)AAAG sequence as the recognition core. It suggests that the Dof transcription fac-tors play diverse roles in specific biological processes in plants. Members of this protein family ...
OBP4 protein enrichment was indeed detected at the promoter regions of the CYCB1;1, CDKB1;1, XTH9, and XTH17 genes containing the predicted DOF binding sites (Fig. 7B). In sum, we conclude that OBP4 can transcriptionally regulate both cell cycle progression and cell expansion by ...
(Yanagisawa, 2002;Noguero et al., 2013;Gupta et al., 2015). The highly conserved Dof domain is usually located at the N-terminus which includes approximate 52 amino acids with a C2C2zinc finger structure. The Dof domain is essential for specifically binding a conversed target DNA sequence ...
After the first isolation of a complementary DNA from maize encoding a Dof domain-containing protein, these transcription factors have been identified in a variety of angiosperms including Arabidopsis, barley, pea, potato, pumpkin, rice, tobacco and wheat.1 Thereafter, Dof-like genes were identified...