DNase-seq 已经被广泛应用在各个物种中,可靠性得到了很好的验证。 定向切割开放区域内的DHS位点,而不会切割受保护的区域,可以通过DNase-seq 推测核小体可能的位置,和染色质开放性的变化。 DNase-seq需要数以百万计的细胞作为样品,现在已经开发了单细胞的DNase-seq,但无法区分致密的染色质区域和测序过程中丢失的情况...
该视频主要介绍了DNase-seq,即脱氧核糖核酸酶I高敏感位点测序,是分子学领域用来里用来判定调控区位置的方法,基于全基因组范围内对DNase I能够酶切的敏感区域的测序,DNase-seq能够在单核苷酸水平鉴定转录因子起始位点。, 视频播放量 720、弹幕量 0、点赞数 13、投硬币枚数
DNase-Seq: Boyle A. P. et al. (2008) High-resolution mapping and characterization of open chromatin across the genome. Cell 132: 311-322 1. Anderson S. Shotgun DNA sequencing using cloned DNase I-generated fragments. Nucleic Acids Res. 1981;9:3015-3027 ...
DNase-seq: 限制性内切酶DNaseI,对样品进行片段化处理,切割开放区域的DNA。同时,在开放区域,缠绕在核小体上的DNA被核小体结构所保护,只有核小体间的DNA序列可以被DNaseI切割,这些位点也叫DHS。 MNase-seq: (MNase微球菌核酸酶):同时具有核酸外切酶和内切酶活性,优先对裸露的DNA或核小体之间起连接作用的DNA进行切...
• DNase-Seq、ATAC-Seq、FAIRE-Seq 都是用来研究开放染色质区域。 DNase-Seq 是用的 DNase I 内切酶识别开放染色质区域,而 ATAC-seq 是用的 Tn5 转座酶,随后进行富集和扩增;FAIRE-Seq 是先进行超声裂解,然后用酚氯仿富集。 • MNase-Seq 是用来鉴定核小体区域。 ATAC-Seq 简介 ATAC-seq(Assay for Tran...
具体如下: 首先,向我们了解到的,CHIP-seq即染色质免疫共沉淀质谱技术,它主要通过对已知的蛋白,通常是目的转录因子TF免疫沉淀,抓取与该TF结合的DNA片段,然后再进一步的扩增,找到TF的潜在靶基因。通... 查看原文 小鼠植入前胚胎的可接近染色质图谱 开放染色质的allelic图谱TheUCSC browser view shows allelicATAC-seq...
1.一种快速简便构建植物DNase-seq文库的方法,其特征在于,将纯化的植物DNA片段采用AMPure beads首先去除1000bp以上的大片段,然后利用醇沉的方法回收所有小片段用于PCR建库,建库后再一次AMPure beads片段筛选去除插入片段大于200bp的DNA用于测序;通过两轮的AMPure beads筛选,获得用于测序的DNase-seq文库。 2.根据权利要求...
XL-DNase-seq may help extract novel gene regulatory circuits involving previously undetectable TFs. The DNase-seq and ATAC-seq data generated in our systematic comparison of various crosslinking conditions also represent an unprecedented-scale resource derived from activated mouse macrophage-like cells ...
the DNA-protein complexes are stabilized by formaldehyde crosslinking before DNase I digestion. The crosslinking is reversed before DNA purification. In an alternative modification, called GeF-seq, both the crosslinking and the DNase I digestion are carried outin vivo, within permeabilized cells(Chums...
DNase-seq Active enhancer sequences are usually located in NDRs and are therefore sensitive tonucleaseactivity[82]. Therefore, DNase-seq is a very valuable tool to identifycis-regulatory sequences. With this method,DNaseI hypersensitive sites (DHSs) can be identified by partial digestion of ...