DNase-seq (DNase I hypersensitive sites sequencing) is a technique that uses both traditional DNase I footprinting and next-generation sequencing to identify regulatory elements that interact with DNA, regulating chromatin structure and genomic regions (He et al., 2014). ...
此外，在对染色质进行低DNaseI处理，可发现酶切割将先发生在少数特异性位点上，这些特异性位点叫做DNaseI超敏感位点（DNaseI Hypersensitive Sites，DHSs ），它实际上是一段长约200bp的DNA序列特异暴露的染色质区域，甲基化程度较低，富含HMG14,HMG17蛋白，一般在转录起始附近或者相关部位。
DNase I–hypersensitive sites enhance α1(I) collagen gene expression in hepatic stellate cells☆ Author links open overlay panelYutaka Yata 1, Andrew Scanga 1, Andrea Gillan 1, Liu Yang 1, Shimon Reif 1, Michael Breindl 2, David A. Brenner 1,3, Richard A. Rippe 1...
DNase I hypersensitive sites (DHSs) provide important information on the presence of transcriptional regulatory elements and the state of chromatin in mammalian cells1,2,3. Conventional DNase sequencing (DNase-seq) for genome-wide DHSs profiling is limited by the requirement of millions of cells4,...
We have used DNase I as a probe to examine the chromatin structure of mouse immunoglobulin kappa light chain genes in rearranged and unrearranged chromosomes--i.e., in nuclei from myeloma cells and from brain and liver cells. Tissue-specific DNase I-hypersensitive sites are observed 0.7 and 1....
同样,在开放区域,缠绕在核小体上的 DNA 被核小体结构所保护,只有核 小体之间的 DNA 序列能够被 DNase I 切割,这些区域内能够被 DNase 切割的位 点也被称为 DHS,即 DNase 超敏感位点(DNase hypersensitive sites)。 该测序方式的特点: ➢ DNase-seq 已经被广泛应用在各个物种中,可靠性得到了很好的验证。
DNase I hypersensitive sites that are significantly mutated in breast cancers and associated with the aberrant expression of neighboring genes. A pan-cancer analysis shows that three of these elements are significantly mutated across multiple cancer types and have mutation densities similar to protein-...
In genetics, DNase I hypersensitive sites (DHSs) are regions of chromatin that are sensitive to cleavage by the DNase I enzyme. In these specific regions of the genome, chromatin has lost its condensed structure, exposing the DNA and making it accessible. ...
甲基化程度较低，富含HMG14,HMG17蛋白。一般在转录起始附近或者相关部位。全景图 DNase I超敏感位点(DNase I hypersensitive sites, DHSs)是调节性DNA序列的标记物。研究人员通过对125个不同的细胞和组织类型进行全基因组谱分析而鉴定出大约290万个人DHSs，并且首次大范围地绘制出人DHSs图谱。