DNase-seq (DNase I hypersensitive sitessequencing) is a technique that uses both traditional DNase I footprinting and next-generation sequencing to identify regulatory elements that interact with DNA, regulating chromatin structure and genomic regions (He et al., 2014). DNase-seq has been used ...
DNase I hypersensitive site (DHS) mapping combined with high‐throughput sequencing (DNase﹕eq) enables the identification of cis‐regulatory DNA elements (CREs) genome wide. However, despite the wide applications of DNase﹕eq in plants, its application to the highly repetitive genomes of plants ...
DNase I hypersensitive sites (DHSs) are regions of chromatin that are sensitive to cleavage by the DNase I enzyme. In these specific regions of the genome, chromatin has lost its condensed structure, exposing the DNA and making it accessible. This raises the availability of DNA to degradation ...
1. Anderson S. Shotgun DNA sequencing using cloned DNase I-generated fragments. Nucleic Acids Res. 1981;9:3015-3027 2. Jin W., Tang Q., Wan M., et al. Genome-wide detection of DNase I hypersensitive sites in single cells and FFPE tissue samples. Nature. 2015;528:142-146 ...
主要是ChIP-seq(immunological assays)占了很大一类,把它搞懂就行。 另一类non-immunological assays:ATAC-seq, MNase-seq, DNase-seq, and FAIRE-seq。 DHSs DNase I hypersensitive site DNase-seq FAIRE-Seq is a successor genome-wide DNA footprints ...
DNase I hypersensitive sites (DHSs) provide important information on the presence of transcriptional regulatory elements and the state of chromatin in mammalian cells1,2,3. Conventional DNase sequencing (DNase-seq) for genome-wide DHSs profiling is limited by the requirement of millions of cells4,...
Conclusion: This protocol simplifies and facilitates generation of DNase-seq libraries from plant tissues for high reso- lution mapping of DNase I hypersensitive sites. Keywords: DNase-seq, DNase I hypersensitive sites, Open chromatin, Arabidopsis, Roots, Nuclei Backgroun...
NIH 1992F32 GM DNA Structure of A Dnase I Hypersensitive SiteTeare, John M. / Washington University NIH 1991F32 GM DNA Structure of A Dnase I Hypersensitive SiteTeare, John M. / Washington University NIH 1990F32 GM DNA Structure of A Dnase I Hypersensitive SiteTeare, John M. / Washin...
insulator element/DNase I hypersensitive site I in three popu- lations of HeLa cells: ‘‘asynchronous cells’’ (primarily inter- phase), ‘‘mitotic cells’’ (arrested with nocodazole), and ‘‘released cells’’ (incubated for 5 h after release from mitotic arrest). The cells were permeab...
DNase I hypersensitive sites that are significantly mutated in breast cancers and associated with the aberrant expression of neighboring genes. A pan-cancer analysis shows that three of these elements are significantly mutated across multiple cancer types and have mutation densities similar to protein-...