2020年6月29日,Nature Plants在线发表了浙江大学李正和教授研究组题为Highly efficient DNA-free plant genome editing using virally delivered CRISPR-Cas9 的研究论文。该研究首次报道了利用一种工程化的植物负链RNA弹状病毒载体向植物体内系统递送CRISPR/Cas9核酸内切酶,并高效产生可稳定遗传的靶向基因编辑。值得一提...
2020. CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off-Target Evaluation, and Strategies to Mitigate Off-Target Effects. Adv Sci (Weinh) 7:1902312. Toda E, Koiso N, Takebayashi A, Ichikawa M, Kiba T, Osakabe K, Osakabe Y, Sakakibara H, Kato N, Okam...
To alleviate concerns related to genetically modified plants, the present disclosure provides a novel and efficient genome editing system that allows the regeneration of mutant plants without DNA stable integration. This DNA free system utilizes Cas9 mRNA, guide RNA and selectable marker RNA to infect...
Manghwar H, Li B, Ding X, Hussain A, Lindsey K, Zhang X, Jin S. 2020. CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for sgRNA Design, Off-Target Evaluation, and Strategies to Mitigate Off-Target Effects. Adv Sci (Weinh) 7:1902312. Toda E, Koiso N, Takebayashi A,...
Chen K, Wang Y, Zhang R, Zhang H, Gao C. 2019. CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture. Annu Rev Plant Biol 70:667-697. Gao C. 2021. Genome engineering for crop improvement and future agriculture. Cell 184:1621-1635. ...
Progress and prospects in plant genome editing. Nature Plants. 2017, 3: 17107. 植物DNA-free基因编辑,植物DNA-free基因编辑 植物DNA-free基因编辑信息由北京安必奇生物科技有限公司为您提供,如您想了解更多关于植物DNA-free基因编辑报价、型号、参数等信息,欢迎来电或留言咨询。 注:该产品未在中华人民共和国食品...
Genome-editing technologies based on the CRISPR (clustered regularly interspaced short palindromic repeat) system have been widely used in plants to investigate gene function and improve crop traits. The recently developed DNA-free delivery methods and precise base-editing systems provide new opportunities...
Optimizing multiplex DNA-free genome editing Our main objective was to inactivate multiple genes of interest in a single step, relying on thePtUMPSKO 5-FOA-resistance phenotype for positive selection. We started with the assumption that double KO strains could be generated by bombarding cells with...
Genome-wide analysis reveals specificities of Cpf1 nucleases in human cells. Nat. Biotechnol. 34, 863–868 (2016). Article CAS Google Scholar Woo, J. W. et al. DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins. Nat. Biotechnol. 33, 1162–1164 (2015). ...
DNA-free genome editing methods for targeted crop improvement Evolution of the next-generation clustered, regularly interspaced, short palindromic repeat/Cas9 (CRISPR/Cas9) genome editing tools, ribonucleoprotein (RNA... Kanchiswamy,Chidananda Nagamangala - 《Plant Cell Reports》 被引量: 15发表: 2016...