To alleviate concerns related to genetically modified plants, the present disclosure provides a novel and efficient genome editing system that allows the regeneration of mutant plants without DNA stable integration. This DNA free system utilizes Cas9 mRNA, guide RNA and selectable marker RNA to infect...
2020年6月29日,Nature Plants在线发表了浙江大学李正和教授研究组题为Highly efficient DNA-free plant genome editing using virally delivered CRISPR-Cas9 的研究论文。该研究首次报道了利用一种工程化的植物负链RNA弹状病毒载体向植物体内系统递送CRISPR/Cas9核酸内切酶,并高效产生可稳定遗传的靶向基因编辑。值得一提...
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Ma X, Zhang X, Liu H, Li Z. 2020. Highly efficient DNA-free plant genome editing using virally delivered CRISPR-Cas9. Nat Plants 6:773-779. Manghwar H, Li B, Ding X, Hussain A, Lindsey K, Zhang X, Jin S. 2020. CRISPR/Cas Systems in Genome Editing: Methodologies and Tools for...
Genome-editing technologies based on the CRISPR (clustered regularly interspaced short palindromic repeat) system have been widely used in plants to investigate gene function and improve crop traits. The recently developed DNA-free delivery methods and precise base-editing systems provide new opportunities...
Multiple gene knock-outs using a DNA-free RNP genome editing approach.aOutline of the genome-editing workflow used here. The first step consists of generating four independent RNP complexes, two of them targetingPtUMPSand the two others targetingPtAureo1aor any other gene of interest. Each RNP...
Cpf1, a type V CRISPR effector, recognizes a thymidine-rich protospacer-adjacent motif and induces cohesive double-stranded breaks at the target site guided by a single CRISPR RNA (crRNA). Here we show that Cpf1 can be used as a tool for DNA-free editing
DNA-free genome editing in tomato protoplasts using CRISPR/Cas9 ribonucleoprotein delivery CRISPR/Cas9 ribonucleoproteins enable DNA-free genome editing; thus, improving protoplast culture is essential for the efficient development of mutant plan... GH Kang,BC Kang,JSJ Min - 《Horticulture Environment &...
Enhanced base editing by co- expression of free uracil DNA glycosylase inhibitor[J]. Cell Res, 2017, 27(10): 1289-1292. [35]Gehrke JM, Cervantes O, Clement MK, et al. An APOBEC3A-Cas9 142 生物技术通报 Biotechnology Bulletin 2024,Vol.40,No.1 base editor with minimized bystander and ...