cDNA libraryA cDNA library represents a collection of only the genes that are encoded into proteins by an organism. Complementary DNA, or cDNA, is created through reverse transcription of messenger RNA, and a library of cDNAs is generated using DNA cloning technology. ...
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point mutationThe effect of base substitutions, or point mutations, on the messenger-RNA codon AUA, which codes for the amino acid isoleucine. Substitutions (red letters) at the first, second, or third position in the codon can result in nine new codons corresponding to six different amino aci...
2). Transcription is catalyzed by RNA polymerase and is initiated at a DNA sequence referred to as the promoter. Ribonucleic acid (RNA) is comparable to DNA but consists of nucleotides in which the sugar is ribose, and the pyrimidine base, T, is replaced by another pyrimidine base, uracil ...
Repetitive DNA sequences playing critical roles in driving evolution, inducing variation, and regulating gene expression. In this review, we summarized the definition, arrangement, and structural characteristics of repeats. Besides, we introduced diverse
Single-stranded RNA virus genomes may be positive-sense (i.e., the same polarity or nucleotide sequence as the mRNA; designated as ssRNA+), negative-sense (opposite polarity; ssRNA−, needs to be transcribed for mRNA), or ambisense (a mixture of the two, with an ssRNA− replication st...
A method for enriching whole genomic DNA for many regions of interest by hybridization to a complex library of RNA or DNA sequences in solution, followed by retrieval of the annealed hybrids. Hemimethylated Methylation of a residue on one strand within a palindromic target sequence but not of ...
Definition 3. A GenModel M is defined as a directed graph [Math Processing Error]G=C,Ewhere the vertices are the set of clusters [Math Processing Error]C={C1,C2,...,Cm}of NSVs and the edges E represent the ordering of the NSVs in the sequences. Each cluster contains metadata such...
Although H-NS dramatically affects transcription, an elongating RNAP can also remodel an H-NS or H-NS:StpA filament[101,102]. Elongating RNAP may encounter an H-NS filament in acoding region[54]or downstream of an antisense promoter[103], especially when ρ-dependent termination is suppressed...
Both E. coli and T4 DNA ligases join 5′-P DNA to 3′-OH RNA in the presence of the complementary DNA strand. The joining in reverse combination, i.e., 5′-P RNA to 3′-OH DNA, is catalyzed by the T4 enzyme only (9, 10). iv. RNA substrates. The T4 DNA ligase, but not ...