Ana Maria Ulhoa Escobar3, Carlos Alberto Moreira-Filho3, Guilherme Vanoni Polanczyk2, Euripedes Constantino Miguel2 & Helena Brentani2 Sex differences in the prevalence of psychiatric disorders are well documented, with exposure to stress during gestation differentially impacting ...
by JEV-infected mouse brain supernatant or RNA interference by targeting Afdn gene for the experiment setup: (1) to determine role of JEV supernatant treatment in cell permeability, bEnd.3 was treated by JEV supernatant and mock supernatant, respectively; (2) to determine role of Afdn in cell ...
Spermatozoa are the cells that are most commonly used for cryopreservation of valuable genetic resources in aquaculture. It is known that fish spermatozoa transmit to the embryo not only their genetic but also their epigenetic profile, especially DNA met
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Epigenetic variation includes DNA methylation, histone modifications, and RNA-mediated gene silencing [76]. DNA methylation refers to the transfer of a methyl group onto the 5thposition of the cytosine to form 5 methyl cytosine (5mC) [61]. In plants, DNA methylation typically occurs at cytosine...
Early detection of hepatocellular carcinoma (HCC) is important in order to improve patient prognosis and survival rate. Methylation sequencing combined with neural networks to identify cell-free DNA (cfDNA) carrying aberrant methylation offers an appeali
To make mRNA, RNA polymerase: Binds to the DNA strand at a specific sequence of the gene called a promoter Unwinds and unlinks the two strands of DNA Uses one of the DNA strands as a guide or template Matches new nucleotides with their complements on the DNA strand (G with C, A with...
After PCR amplification, uracil bases are changed over to thymine. DNA methylation status can be dictated via direct PCR sequencing [60]. Since the estimated level of DNA methylation depends on complete conversion of non-methylated cytosine residues, we need to make sure of this process. Thus, ...
We find that the RNA N6-methyladenosine (m6A) reader, YTHDC2, occupies genomic loci of the primate-specific TE, LTR7/HERV-H, specifically through its interaction with m6A-modified HERV-H RNAs. Unexpectedly, YTHDC2 recruits the DNA 5-methylcytosine (5mC)-demethylase, TET1, to remove 5mC ...
127, 128, 129 Single-cell analysis of DNA methylation is technically difficult because bisulfite conversion is a relatively harsh process that causes DNA to be randomly fragmented.130 Although single-cell genome-wide BS technologies have been developed, more clinically available DNA methylation analysis...