Characterisation of viruses comprises (1) converting viral RNA to cDNA; (2) amplifying the cDNA in presence of primers 1 and 2, from the region of sequence identity, using the polymerase chain reaction; (3) mapping the amplified DNA using various restriction enzymes, and (4) comparing the ...
A polymorphism consisting of the presence or absence of a 250-bp DNA fragment was detected within the angiotensin I-converting enzyme gene (ACE) using the ... B Rigat,C Hubert,F Alhenc-Gelas,... - 《Journal of Clinical Investigation》 被引量: 6477发表: 1990年 Cardiac overexpression of ...
Converting Messenger RNA into Complementary DNAconverting messenger RNA into complementary DNAviral reverse transcriptionextracting messenger RNA (mRNA) from cells and tissuesfirst strand synthesisviral reverse transcriptionsecond strand reactionsreverse transcriptase PCR...
摘要: Herein we report that Aβ aggregates, not Aβ monomer, can convertZ-DNA back to B-form, and curcumin, a well-known Aβ aggregation inhibitor, can stop DNAZ-B transition studied by means of biophysical and biochemical methods.关键词:...
The E3L gene product consists of an N-terminal Zα domain and a C-terminal double-stranded RNA (dsRNA) binding domain; the left-handed Z-DNA-binding activity of the Zα domain of E3L is required for viral pathogenicity in mice. E3L is highly conserved among poxviruses, including the ...
Small RNA-mediated chromatin silencing is well characterized for repeated sequences and transposons, but its role in regulating single-copy endogenous gene... S Swiezewski,P Crevillen,F Liu,... - 《Proc Natl Acad Sci U S A》 被引量: 289发表: 2007年 A DNA motif related to the cAMP-re...
For example, the bacterial glycine riboswitch facilitates glycine breakdown by controlling the expression of three genes required for degradation in response to glycine binding3. Similar target-responsive RNA- and DNA-based molecular switches have potential utility in a variety of technological applications...
Overlapping genomic clones containing the entire sequence of the human angiotensin I-converting enzyme (ACE) gene were isolated from a lamda phage human DNA library. This gene spans 21 kilobases (kb) and comprises 26 exons, ranging in size from 88 to 481 base pairs. Intron-exon boundaries were...
Effects of insertions and deletions in glnG (ntrC) of Escherichia coli on nitrogen regulator I-dependent DNA binding and transcriptional activation. Phosphorylated nitrogen regulator I (NRI, also called NTRC), encoded by glnG (ntrC), stimulates transcription in Escherichia coli and other enteric bacte...
An important application of robotically spotted DNA microarrays is the monitoring of RNA expression levels1,2. A clear limitation of this technology is the relatively large amount of RNA that is required per hybridization as a result of low hybridization