In addition to HF prevention, understanding the prognosis of patients with HF is also needed [7]. Indeed, while some patients experience prolonged periods without hospitalization, others face an unstable clinical course with frequent HF decompensation and a poor prognosis [31]. The Seattle Heart Fai...
4a–f). Substrate unfolding by p97 was monitored by fusing I3 to mEos (forming I3mEos)33. mEos is a photoactivable protein that shifts its fluorescence emission from green to red upon the ultraviolet (UV)-induced breakage of a peptide backbone near the chromophore34. This prevents mEos ...
the differential pathogenesis of polyglutamine disorders may be due to differences in polyglutamine protein context or the host protein function. This is despite nearly ubiquitous expression of these proteins, at least in the brain, and, in the case ofHTT, expression throughout the body and during...
aHe also created home study courses for stocks and commodities and taught weekend seminars to explain the use of special price and time calculator tools he had invented. These materials were considered valuable enough that in 1932 people were paying $1,500 for his home study commodity course, an...
peptide sequences on the basis of sequence length and average base/amino acid molecular weights. Also includes a list of 1200 of the most commonly encountered organic and inorganic compounds, which may be immediately selected in order to calculate their molecular weights, as well as an easy-to-...
Researchers from MIT applied theRBS Calculatorto optimize the expression of five antimicrobial peptides, delivered using phagemids that target pathogenic bacteria inside the human body. Krom, R. J., Bhargava, P., Lobritz, M. A., & Collins, J. J. (2015). Engineered phagemids for nonlytic...
Molecular Weight Calculator In vivo Batch: Add solvents to the product individually and in order. In vivo Formulation Calculator Clear solution 5% DMSO195% Corn oil 0.18mg/ml (0.58mM)Taking the 1 mL working solution as an example, add 50 μL of 3.6 mg/ml clear DMSO stock solution to 950...
(rbs). The vector also carries the pBR322-derived ColE1 replicon,lacIgene and ampicillin resistance gene. This vector can be transformed into the same cell withpACYCDuet™-1,pCDFDuet™-1, andpRSFDuet™-1orpCOLADuet™-1in the appropriate host strain for the coexpression of up to eight...
42.A kit for authenticating whether a plant sample is originated from a Chinese medicinal plant ofIlex asprella, Ilex latifoliaorIlex rotunda, comprising:an array prepared by the steps of:a) extracting DNAs from the plant;b) amplifying ITS1 and ITS2 regions from the extracted DNAs to obtain...
1.A method of cloning DNA using T4 DNA polymerase comprising:a) combining linear DNA fragments having terminal sequence homologies between 15 and 100 nucleotides;b) generating single-stranded recesses by adding the T4 DNA polymerase to the linear DNA fragments; andc) treating the T4 DNA polymerase...