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The Innovagen Peptide solubility calculator was used to predict water solubility24. Lyophilized custom-ordered peptides were reconstituted in three different buffers: (i) 5% ACN in AmBic/SDC, (ii) 20% ACN in AmBic/SDC, and (iii) 50% ACN in AmBic/SDC. Stock solutions (10 µM) were ...
as indicated in the following. A.Z. developed deep learning codes and simulations and wrote the corresponding method section and performed BLAST analysis. A.P. and Y.F. performed cell-free protein synthesis and the initial bioactivity tests. M.K. performed the RBS calculator and kinfold calcula...
(MS/MS) were employed with the Orbitrap Velos Pro. Scoring parameters were optimized to provide a peptide false discovery rate of 1% or less. The identified peptides were processed using MS Excel and online software to create Venn diagrams [16] and calculate the grand average of hydropathicity...
IPC–Isoelectric Point Calculator. Biol. Direct 2016, 11, 1–16. [Google Scholar] [CrossRef] [PubMed] [Green Version] Hyde, A.M.; Zultanski, S.L.; Waldman, J.H.; Zhong, Y.-L.; Shevlin, M.; Peng, F. General principles and strategies for salting-out informed by the Hofmeister ...
In Silico Analysis The "APD3: Antimicrobial Peptide Calculator and Predictor" tool of the Antimicrobial Peptide Database (APD) [18] was used to identify the following general characteristics of antimicrobial peptides, such as hydrophobicity, affinity to attach to other proteins (Boman index) and ...