riceSingle-stranded DNA (ssDNA) is essential for various DNA-templated processes in both eukaryotes and prokaryotes. However, comprehensive characterizations of ssDNA still lag in plants compared to nonplant systems. Here, we conducted in situ S1-sequencing, with starting gDNA ranging from 5 g to ...
DNA-templated synthesis: More versatile than expected - Summerer, Marx () Citation Context ...n processes. Interestingly, it appears that the DNA acts in a similar manner to enzymes and promotes chemical reactions through spatial alignment of the reaction partners; i.e., via a template effect...
A biohybrid, leaf-spring design of DNA origami functions as a pulsating nanoengine that exploits the DNA-templated RNA transcription mechanism while consuming nucleoside triphosphates as fuel. The nanoengine also drives a nanomechanical follower structure. ...
F. Rolling circle amplification-templated DNA nanotubes show increased stability and cell penetration ability. J. Am. Chem. Soc. 134, 2888–2891 (2012). Article CAS Google Scholar Jensen, S. A. et al. Spherical nucleic acid nanoparticle conjugates as an RNAi-based therapy for glioblastoma. ...
Such a state guarantees its appropriate condensation inside the cell and also helps to regulate various DNA-templated processes, for example, by facilitating the local melting of the DNA helix during transcription, replication, or recombination [96]. Cellular DNA topology has been reported to depend...
Nucleic-acid-templated synthesis as a model system for ancient translation Researchers have used the first approach to perform up to three reactions on a singleDNAtemplate (Figure 4a). For example, Gartner, Liu and co-workers used aDNA templatedisplaying aprimary aminegroup on the non-Watson–Cr...
and self-organization within tissues. Originating from spatiotemporally organized structures and machinery, coupled with information processing in signalling networks, cells embody the ‘sensor–processor–actuator’ paradigm. Can we glean insights from these processes to construct primitive artificial systems ...
(EGFR) onto a 2D DNA origami nanosheet50. In addition to building connections directly, the concept of DNA-templated protein conjugation (DPTC) was proposed based on reversible His-tag-Ni2+-NTA-DNA conjugation51. A complementary DNA strand carrying a reactive group, e.g., N-hydroxysuccinimide...
“Negative regulation of transcription DNA-templated" (P = 1 × 10−4), “Positive regulation of tyrosine phosphorylation of Stat3 protein” (P = 0.020), “Negative regulation of transcription from RNA polymerase II promoter” (P = 0.031), “Regulation of cell shape” (P...
The recognition of distinctly modified histones by specialized ‘effector’ proteins constitutes a key mechanism for transducing molecular events at chromatin to biological outcomes1. Effector proteins influence DNA-templated processes, including transcription, DNA recombination and DNA repair; however, no eff...