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Replication in eukaryotes starts at multiple origins of replication. The mechanism is quite similar to that in prokaryotes. A primer is required to initiate synthesis, which is then extended by DNA polymerase as it adds nucleotides one by one to the growing chain. The leading strand is synthesize...
Most of our knowledge concerning the mechanism and enzymology of eukaryotic DNA replication has been derived from animal cell systems (25,33,39,41,44,9,12,47) although simple eukaryotes, like yeast, offer some advantages. A haploid cell of Saccharomyces cerevisiae contains only three times as ...
In eukaryotes, the leading strand DNA polymerase Polε synthesises the DNA with higher processivity when in complex with the DNA clamp PCNA. Here, the authors report two cryo-EM structures of human Polε bound to the PCNA clamp and a DNA substate, revealing the conformational changes associated...
In eukaryotes, the vast bulk of DNA synthesis takes place during the S phase of the cell cycle, and the entire genome must be unravelled and replicated in order to produce two daughter copies of the organism. It takes three essential phases for DNA replication to occur: first, the opening...
Replication fork reversal in eukaryotes: from dead end to dynamic response. Nat. Rev. Mol. Cell Biol. 16, 207–220 (2015). Article CAS PubMed Google Scholar Mayle, R. et al. Mus81 and converging forks limit the mutagenicity of replication fork breakage. Science 349, 742–747 (2015)....
Translesion DNA synthesis in eukaryotes: a one- or two-polymerase affair Genes Dev., 16 (2002), pp. 1872-1883 CrossrefView in ScopusGoogle Scholar Rattray and Strathern, 2003 A.J. Rattray, J.N. Strathern Error-prone DNA polymerases: when making a mistake is the only way to get ahead ...
error-free. In eukaryotes, DNA damage tolerance involves a error-free pathway dependent on homologous recombination and a more mutagenic pathway based on TLS polymerases [11]. In this review we focus on the contribution of TLS polymerases to DNA damage tolerance and their relevance in cancer ...
DNA Synthesis Inhibition refers to the process where the replication of DNA is temporarily halted in response to DNA damage or other cellular signals, aiming to prevent the formation of new DNA strands until the existing issues are resolved. ...
Also, delocalization of Ulp1 caused by mutations in the Nup84 complex results in DNA damage sensitivity36 but how Ulp1-associated NPCs safeguard genome integrity is poorly understood. In eukaryotes, breaks within repeated sequences (Heterochromatin, rDNA) shift away from their chromatin environment, ...