This chapter discusses components of nucleotides, DNA, and protein synthesis. Genetic information is stored in DNA in the nucleus and mitochondria of cells. DNA consists of two strands of nucleotides on a phospho deoxyribose backbone. The two strands form a double helix that is stabilized by the...
DNAandProteinSynthesis NucleicAcids NucleicAcids-Function •Controltheprocessesof hereditybywhichcellsand organismsreproduceproteins. NucleicAcids–Types •DNA –DeoxyribonucleicAcid •RNA –RibonucleicAcid NucleicAcid–Structure •MadeupofachainofNucleotidesthat ...
Communication between DNA polymerases and Replication Protein A within the archaeal replisome The single stranded DNA binding protein RPA plays a pivotal role in DNA replication. The archaeal RPA hosts a WH domain that interacts with the DNA primase and the replicative polymerase PolD. Here the au...
Nucleic Acids and Protein Synthesis All functions of a cell are directed from some central form of information (DNA). This "biological program" is called the Genetic Code. This is the way cells store information regarding their structure and function. Composition and Structure History of DNA Compo...
Cyanuric acid, a small molecule with three thymine-like faces, reprogrammes the assembly of unmodified poly(adenine) into long fibres with a unique internal structure. The association of adenine and cyanuric acid units into a hexameric rosette motif brings together poly(adenine) triplexes with subse...
Protein synthesis is a two step process.DNA —(transcription)→ RNA —(translation)→ PolypeptideTranscription happens when the information from the DNA template is transcribed onto another form of nucleic acid known as ribonucleic acid or RNA (actually messenger RNA)....
There are two major classes of chemical probes of DNA structure: those that demonstrate no inherent base preference but are sensitive to DNA geometry, such as groove widths and chirality, and those that are base-specific, detecting bases in distorted regions of the helix. The former bind to DN...
基于新生DNA测序的eSPAN（enrichment and sequencing of protein-associated nascent DNA）实验分析表明，破坏Mcm2-NTE与Tof1的相互作用会严重影响亲代组蛋白回收和递送至新生成滞后链DNA的比例。这项工作清晰的展示了DNA复制偶联的亲代组蛋白回收过程中一个关键中间步骤的分子细节，阐明了FACT、Tof1、Mcm2等在亲本组...
论文进一步设计了相应的突变株,以干扰Mcm2-NTE和Tof1的结合。基于新生DNA测序的eSPAN(enrichment and sequencing of protein-associated nascent DNA)实验分析表明,破坏Mcm2-NTE与Tof1的相互作用会严重影响亲代组蛋白回收和递送至新生成滞...
DNA synthesis assessed in axenic media supports the limited protein biosynthesis data We then tested if the axenic media also promoted DNA synthesis (Fig.7).3H thymidine incorporation was measured in the axenic media along with35S Cys-Met incorporation to assess DNA and protein synthesis, respectivel...