Self-assembly of DNA rings from scaffold-free DNA tiles We report a scaffold-free approach in which four- and six-helix DNA bundle units, assembled from a small number of single stranded DNA oligonucleotides pre... Zhang,Nangreave,Yang,... 被引量: 0发表: 0年 Self-assembly of DNA into ...
Enzyme AssemblyEnzymes are often spatially organized within the cell,either in the oligomeric state orin close proximity on the cell membrane or confined in a micro-compartment.Thoseenvironments are believed to play key roles in enabling the high efficiency and specificityof sequential metabolic ...
& Yan, H. Organizing DNA origami tiles into larger structures using preformed scaffold frames. Nano Lett. 11, 2997–3002 (2011). ADS Google Scholar Woo, S. & Rothemund, P. W. K. Self-assembly of two-dimensional DNA origami lattices using cation-controlled surface diffusion. Nat. Commun....
Controlled co-reconstitution of multiple membrane proteins in lipid bilayer nanodiscs using DNA as a scaffold. ACS Chem. Biol. 10, 2448–2454 (2015). Article CAS Google Scholar Niemeyer, C. M., Sano, T., Smith, C. L. & Cantor, C. R. Oligonucleotide-directed self-assembly of proteins...
The assembly of the DNA-PK complex at DNA ends is also required for the NHEJ ligation step. Required to protect and align broken ends of DNA. May also act as a scaffold protein to aid the localization of DNA repair proteins to the site of damage. Found at the ends of chromosomes, ...
De novosequencing usually relies on a hybrid approach for assembling the genome: reads from long-insert paired-end sequencing, referred to asmate-pair sequencing(with higher error rate), are used to build a scaffold, and reads f...
Cells, the fundamental units of life, orchestrate intricate functions — motility, adaptation, replication, communication, and self-organization within tissues. Originating from spatiotemporally organized structures and machinery, coupled with informatio
16.6.1.1.1 Genome sequence and assembly methods For genome sequencing, genomic DNA is derived from the posterior silk glands of day-3 fifth instar larvae of silkworm strain p50T (Daizo). The genomic DNA is fragmented using the HydroShear process (GeneMachines Inc., USA); fragments of 2–3 ...
This pioneering study depicts the method of 2D DNA origami, where a circular ssDNA scaffold and staple strands fold into intricate shapes, such as squares, discs and five-pointed stars. Article CAS PubMed Google Scholar Douglas, S. M. et al. Self-assembly of DNA into nanoscale three-...
Besides, DTF has gained increasing interest owing to its one-pot assembly, simple design, and high-yield attributes.29,30 DTF was used as a scaffold for probes, enabling precise spatial orientation and distance control at the nanometric scale.31 The spatial confinement of DTF can improve the ...