In S phase, initiation of DNA replication by CDK2 promotes helicase activation, which leads to unwinding of DNA and recruitment of DNA polymerases alpha (POLA), delta (POLD), and epsilon (POLE), together with PCNA (proliferating cell nuclear antigen) and RFC (replication factor C). The MCM...
To address whether slow replication dynamics is a feature of genuine totipotent cells, we measured replication fork speed in 2-cell-stage embryos in vivo (Fig. 1e). Notably, 2-cell-stage embryos displayed a low fork speed during their complete S-phase (median 0.33 kb min−1 in ear...
The necessary cell‐cycle regulation is mainly executed at the initiation rather than the elongation step of DNA replication. Our knowledge of mechanisms responsible in eukaryotes largely depends on findings with budding yeast, since identification of specific DNA sequences acting as replication origins ...
DNA replication initiation is a two-step process. During the G1-phase of the cell cycle, the ORC complex, CDC6, CDT1, and MCM2–7 assemble at replication origins, forming pre-replicative complexes (pre-RCs). In S-phase, kinase activities allow fork establishment through (CDC45/MCM2–7/...
replication origin is sufficient to direct all DNA synthesis in prokaryotes. In contrast, eukaryotic chromosome replication requires the orchestration of multiple DNA replication origins to ensure that each DNA polymer, irrespective of its size in the cell, is replicated exactly once during S phase of...
are involved in the initiation phase of SV40DNA replication[6]and we propose that they play a similar role in the DNA synthesome[26]. It was, therefore, proposed thattopoisomerase Iand RPA constitute the “initiation” component of the DNA synthesome. Together, the core and initiation componen...
MRC HGU）的Andrew Jackson研究组题为In silico protein interaction screening uncovers DONSON’s role in replication initiation 的文章（详见今日BioArt解读）；以及，日本东京药科大学的桥本吉民研究组在EMBO J.杂志发文 Novel role of DONSON in CMG helicase assembly during vertebrate DNA replication initiation。
为了探究这一机制,作者利用体外实验系统研究并发现,化学合成的鱼精蛋白能够与双链DNA分子在体外迅速地发生相变分离(Phase transition),形成胶状颗粒(gel-like particle),并逐渐形成致密的交联网络。 如果把这一过程简单地理解为精子发生过程中鱼精蛋白对基因组DNA的压缩排列,那么鱼精蛋白的磷酸化修饰会对其造成何种改变...
Replicon（复制子）isaunitofthegenomeinwhichDNAisreplicated;containsanorigin（复制起点）forinitiationofreplicationandaterminus（复制终点）tostopthereplication.Definitionofreplicon TheunitofDNAinwhichanindividualactofreplicationoccursiscalledthereplicon.Eachreplicon"fires"onceandonlyonceineachcellcycle.Ithasanoriginat...
2022年6月8日,来自美国宾夕法尼亚大学的Jennifer E. Phillips-Cremins团队在Nature杂志上在线发表了题为Cohesin-mediated loop anchors confine the locations of human replication origins的文章。研究人员剖析了TADs、subTADs和环(loops)在复制起始区(ini...