[38].Homoplasmyrestoration was initially explained on the basis of the random choice of anmtDNA templatefor replication and segregation[38]. However, if each mtDNA replicates independently and then randomly segregates into progeny, approximately three mtDNAs should enter into each mitotic cell and ...
Accurate chromosomal DNA replication is essential to maintain genomic stability. Genetic evidence suggests that certain repetitive sequences impair replication, yet the underlying mechanism is poorly defined. Replication could be directly inhibited by th
Besides DNA replication, Dna2 also plays a pivotal role in DSB repair, where it digests the RPA-coated 5′-strand unwound by the Sgs1 helicase to aid DSB end processing9,10,11,12. The Sgs1–Dna2 pair serves as an alternative means in addition to the Exo1 nuclease to catalyze the ...
A more likely mechanism is some form of slipped strand mispairing, whether occuring during normal DNA replication or during replication accompanying recombination. (2) Some results are presented on the number of mono- and di-nucleotide repeats in the human genome. For each high scoring locus, an...
“When WEE1 was inhibited in cells with a SETD2 mutation, the levels of deoxynucleotides, the components that make DNA, dropped below the critical level needed for replication,” noted Oxford’s Andy Ryan. “Starved of these building blocks, the cells die. Importantly, normal cells in the ...
Characterization of an episome produced in hamster cells that amplify a transfected CAD gene at high frequency: functional evidence for a mammalian replication origin. Mol Cell Biol. 1987;7:1740–50. CAS PubMed PubMed Central Google Scholar Ly P, Brunner SF, Shoshani O, Kim DH, Lan W, ...
DNA double-strand breaks (DSBs) can be caused by environmental agents such as radiation and drugs1,2,3and endogenous metabolism such as transcription and replication stress4,5. For example, normal metabolism has been estimated to cause ~1–50 DSBs per human cell per day6,7. Consequently, fas...
DNA-binding proteins perform a variety of important functions in cells, including transcriptional regulation, chromosome maintenance, replication and DNA repair. The interactions between transcription factors (TFs) and their DNA-binding sites are of particular interest, as these interactions control crucial...
DNA–protein crosslinks (DPCs) induced by aldehydes interfere with replication and transcription. Hereditary deficiencies in DPC repair and aldehyde clearance processes cause progeria, including Ruijs–Aalfs syndrome (RJALS) and AMeD syndrome (AMeDS) in humans. Although the elimination of DPC during re...
Positive supercoiling is generated ahead of replication and transcription machinery as they progress along the DNA template42,43,44. Nucleosomes in chromatin limit the intertwining of the newly replicated daughter duplexes behind the polymerase during replication, driving the supercoiling to partition pri...