Discuss the process of DNA replication. Describe the process of DNA replication; include the following terms: antiparallel structure DNA Okazaki fragments, DNA ligase, primer, primase, helicase, topoisomerase, single-strand binding proteins. Describe the differences between prokaryotes and eukaryotes in DN...
Eukaryotic chromosomes are much longer than bacterial ones and have multiple replication origins. Just as in prokaryotes, several proteins load onto the origin of replication in a specific order to control replication initiation in eukaryotes. In eukaryotes the cell cycle consists of a “rest” period...
DNA replication in eukaryotes is a conserved system that only allows for DNA replication to occur once throughout each cell cycle.Share Introduction In the course of DNA replication, a single strand of DNA duplicates itself several times. It is a type of biological polymerisation that occurs in ...
If the action of telomerase in these cells can be inhibited by drugs during cancer therapy, then the cancerous cells could potentially be stopped from further division. Section Summary Replication in eukaryotes starts at multiple origins of replication. The mechanism is quite similar to that in ...
In eukaryotes, the origin recognition complex (ORC) is essential for replication licensing. Here, the authors report that a short motif within the intrinsically disordered region of the yeast Orc2 subunit plays an important role in regulating the licensing process. Yue Wu , Qiongdan Zhang & Yuan...
Understanding replication origins and initiation sites in eukaryotes using ChIP-approaches. Chromosome Res. 18, 63–77 (2010). Article CAS PubMed Google Scholar MacAlpine, H. K., Gordan, R., Powell, S. K., Hartemink, A. J. & MacAlpine, D. M. Drosophila ORC localizes to open ...
The correct processing of Okazaki fragments during lagging-strand DNA replication has a vital role in maintaining genome integrity. Recent findings suggest that, in eukaryotes, the processing of Okazaki fragments occurs by a two-step mechanism governed by the single-stranded DNA binding factor RPA....
The proteins of replication forks of eukaryotes and prokaryotes are also different, but the corresponding parallel relationship can be found, as summarized in the figure below. Fig. 13 Comparison of replication forks between prokaryotes and eukaryotes ...
注:①“非编码损伤”(noncoding or replication-blocking lesions)是指对DNA复制构成明显阻碍的DNA损伤。那些对DNA复制没有明显阻碍但可导致点突变率显著提升的DNA损伤被称为“错码损伤”(miscoding lesions)。(15)本文在讨论TLS时,只涉及“非编码损伤”。
的募集相偶联而真核细胞中这两个事件的短暂分离保证了在每个细胞周期中每条染色体仅仅只复制一次Initiation in eukaryotes requires two distinct steps:1st step---Replicator selection: the process of identifying sequences for replication initiation (G1 phase), which is mediated by the formation of pre-RCs ...