To avoid innate sensing and immune control, human immunodeficiency virus type 1 (HIV-1) has to prevent the accumulation of viral complementary DNA species. Here, we show that the late HIV-1 accessory protein Vpu hijacks DNA repair mechanisms to promote degradation of nuclear viral cDNA in cells...
Cell Host Microbe 27(5):841–848. Resources for Platinum SuperFi II DNA polymerase Flyers Platinum SuperFi II DNA Polymerase Improve microbiome studies Manuals/Protocols Protocol: Site-directed mutagenesis using the GeneArt Gibson Assembly HiFi Cloning Kit Platinum SuperFi II DNA...
Living organisms constantly face environmental challenges. However, being sessile plants cannot avoid the stresses of the natural environment as sensitively as animals. In order to cope with such challenges, they evolved different strategies. One such strategies are the regulation through epigenetic mecha...
Cell Host Microbe 27(5):841–848. Resources for Platinum SuperFi II DNA polymerase Flyers Platinum SuperFi II DNA Polymerase Improve microbiome studies Manuals/Protocols Protocol: Site-directed mutagenesis using the GeneArt Gibson Assembly HiFi Cloning Kit Platinum Sup...
DNA ligase IV: homologous recombination repair and important in immune cell development and other activities critical to a normal cell. NAD+dependent DNA ligases: Bacterial DNA ligases are NAD+dependent ligases. They are also present in some mimivirus and entomovirus. While some bacteria, archaea,...
et al. Target DNA-dependent activation mechanism of the prokaryotic immune system SPARTA. Nucleic Acids Res. 52, 2012–2029 (2024). Article PubMed PubMed Central Google Scholar Shen, Z. et al. Oligomerization-mediated activation of a short prokaryotic Argonaute. Nature 621, 154–161 (2023)....
In addition, this mechanism suggests a protective role of RIG-I in hindering retrovirus integration into the host genome by suppressing the NHEJ pathway. Reciprocally, XRCC4, while suppressed for its DNA repair function, has a critical role in RIG-I immune signaling through RIG-I interaction. ...
Mechanism of resistance of DNA Synthesis Inhibitors Mutations in enzymes may cause quinolone resistance. Genes that cause a chromosomal mutation that codes for protein targets and plasmid-mediated genes. Mutation of serine or acidic residue decreases the affinity of drug and enzyme complex. And disrupti...
The genome-wide DNA methylation profile, or DNA methylome, is a critical component of the overall epigenomic landscape that modulates gene activities and cell fate. Single-cell DNA methylomic studies offer unprecedented resolution for detecting and profi
When it is activated, it creates a checkpoint, stops replication, and gives time to properly repair lesions in the new DNA strand. It is also involved in the repair mechanism of translesion synthesis. It does not have nuclease activity, therefore it is prone to errors in DNA replication. ...