Synopsis By itself, the polymerase catalytic subunit of the DNA polymerase III holoenzyme (Pol III HE), α, exhibits no special properties that hint of the Pol III HE's high catalytic efficiency, accuracy, and enormous processivity. These properties are gained by association with other proteins ...
DNA polymerase III', a new form of DNA polymerase III, has been purified 15,000-fold to 90% homogeneity from an Escherichia coli K12 strain. DNA polymerase III's is a subassembly of four subunits of the DNA polymerase III holoenzyme; it has functional and physical properties intermediate bet...
Structure of the SSB–DNA polymerase III interfaceand its role in DNA replication SSB-DNA聚合酶III接口结构和该结构在DNA复制中的作用 The EMBO Journal(2011)30, 4236–4247 IF=9.205 一. 研究背景及问题的提出 大肠杆菌的DNA复制起始于oriC位点,在该位点形成两个分开的复制叉,多种蛋白质组成复制叉形成复制...
The dimeric structure of the DNA polymerase III holoenzyme couples leading and lagging strand DNA syntheses during replication. Leading strand synthesis (bottom strand) is continuous and processive, but lagging strand synthesis must reinitiate many times during replication of the chromosome. This ...
2013. Preferential D‐loop extension by a translesion DNA polymerase underlies error‐prone recombination. Nat Struct Mol Biol. 20: 748–755. (175)Windgassen TA, Wessel SR, Bhattacharyya B, Keck JL. 2018. Mechanisms of bacterial DNA replication restart. Nucl. Acids Res. 46:504–519. (176)...
The DNA polymerase III ?? subunit (??-clamp) acts as a sliding clamp on DNA, promoting the binding and processivity of many DNA-acting proteins, and here we report the crystal structure of D. radiodurans ??-clamp (Dr??-clamp) at 2.0 ?? resolution. Results The sequence verification ...
2024年8月9日,北京大学生命科学学院、北大-清华生命科学联合中心李晴课题组在Science Advances发表题为DNA polymerase δ subunit Pol32 binds histone H3-H4 and couples nucleosome assembly with Okazaki fragment processing的研究论文,巧妙利用DNA连接酶降解体系,应用电镜核酸技术观察酵母细胞中DNA复制叉中间体结构,发现...
Three-dimensional structure of the beta subunit of E. coli DNA polymerase III holoenzyme: a sliding DNA clamp. Cell. 1992;69(3):425–37. Article CAS PubMed Google Scholar Argiriadi MA, Goedken ER, Bruck I, O’Donnell M, Kuriyan J. Crystal structure of a DNA polymerase sliding clamp...
DNA polymerase ζ (Polζ) belongs to the same B-family as high-fidelity replicative polymerases, yet is specialized for the extension reaction in translesion DNA synthesis (TLS). Despite its importance in TLS, the structure of Polζ is unknown. We present cryo-EM structures of theSaccharomyces...
Fig. 2. DNA polymerase III holoenzyme and replisome structure in E. coli. (A) Illustration of the protein arrangement within the holoenzyme. The subunit names for the core DNA polymerase III, the clamp and the clamp loader with their stoichiometry plus their coding gene and mass in kilodalton...