(1992) The 5′ to 3′ exonuclease activity of DNA polymerase I is essential for Streptococcus pneu- moniae, Mol. Microbiol. 6, 3009Ϫ3019.Díaz A., Lacks S. A., López P. The 5′ to 3′ exonuclease activity of DNA polymerase I is essential for Streptococcus pneumoniae . Mol. ...
DNA Polymerase I (E coli) is a DNA-dependent DNA polymerase with inherent 3´→ 5´ and 5´→ 3´ exonuclease activities. The 5´→ 3´ exonuclease activity removes nucleotides ahead of the growing DNA chain, allowing nick-translation. ...
coli. DNA polymerase I, has DNA polymerase activity, so this enzyme can attach to a short single-stranded region (or nick) in a mainly double-stranded DNA molecule, and then synthesizes a completely new strand (Fig. 11.10B). On the other hand, it also has two different exonuclease ...
DNA polymerase I only contained a double-strand dependent 5′ → 3′ exonuclease activity but lacked any detectable 3′ → 5′ proofreading exonuclease activity. The lack of 3′ → 5′ exonuclease function in a variety of thermostable repair DNA polymerases may reflect enhancement of thermostabilit...
2130BDNA Polymerase I (E.coli)2,500 Units* DNA Polymerase I catalyzes the incorporation of nucleotides into double-stranded DNA in a 5'→3' direction. It also possesses a 3'→5' exonuclease (proofreading) activity. The 5'→3' exonuclease activity is useful for labeling DNA by nick transla...
Polymerase: DNA Polymerase I Product Size: 250 units Exonuclease Activity: 5' - 3', 3' - 5' Shipping Condition: Wet Ice Regulatory Statement: For Research Use Only. Not for use in diagnostic procedures. Store in freezer (-5 to -30°C). ...
DNAPolymeraseactivities 5′-3′exonucleaseactivity:切除修复作用：从5'→3'方向水解DNA生长链前方的DNA链，主要产生5'-脱氧核苷酸。这种酶活性只对DNA上双链磷酸二酯键有切割活力作用，方向是5'→3'。DNA的聚合作用能刺激5'→3'外切酶活力达10倍以上。这种酶活性在DNA损伤的修复中可能起着重要作用。Taq酶的5''...
1、Structure of human DNA polymeraseStructure of human DNA polymerase complexed TTP with manganese in the active site3-5 exonuclease activity: proofreading activity:校正作用:新进入的脱氧核苷酸必须与模板DNA配对时才有催化作用。若是错误的核苷酸进入结合位点,则不能与模板配对,无法改变酶的构象而被3-5...
In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template. 应用类型 Immunoprecipitation (IP), Western blot (WB) 免疫...
M. et al. DNA polymerase epsilon and delta proofreading suppress discrete mutator and cancer phenotypes in mice. Proc. Natl Acad. Sci. USA 106, 17101–17104 (2009). References 11 and 12 show that loss of proofreading exonuclease activity in either Pol δ or Pol ɛ leads to an increase...