histolytica encystation, we examined the level of DNA polymerase activity in E. invadens during encystation induced in vitro. We first characterized the DNA polymerase activity of trophozoites of E. invadens , comparing it with that of E. histolytica , and found that the activity of E. ...
PAXX and XLF form a complex with Pol λ via DNA-bound Ku heterodimers. This interaction requires the C-terminal region of PAXX or XLF. In addition, each XRCC4 family protein stimulates Pol λ-dependent gap-filling activity in vitro via direct interaction between the head domains and the ...
Soluble DNA polymerase (DNA polymerase-α) and chromatin-bound DNA polymerase (DNA polymerase-β) have been assayed in serial sections cut from the roots of 5-day-old pea seedlings. The activity of DNA polymerase-α is high in regions of the root which exhibit high rates of DNA replication...
Differentiation of radioactive DNA and RNA deposited on filter paper discs can be accomplished by a relatively simple procedure. RNA can be efficiently removed by incubating the dises, impaled on pins, with 0.2 ml of 0.5 n NaOH for 90 mi... SC Chou,HYM Pan,P Heu,... - 《Analytical Bio...
A second signal provides valuable complementary information on the polymerase activity: the absolute fluorescence intensity measured for a standing DNA orientation (Fig. 2C,D). In order to align the DNA upright, it is essential to apply a repulsive (negative) surface potential39,41, since otherwis...
coli. DNA polymerase I, has DNA polymerase activity, so this enzyme can attach to a short single-stranded region (or nick) in a mainly double-stranded DNA molecule, and then synthesizes a completely new strand (Fig. 11.10B). On the other hand, it also has two different exonuclease ...
1 in cellular processes such as DNA repair, gene transcription and cell death have allowed the investigation of therapeutic PARP inhibition for a variety of diseases — particularly cancers in which defects in DNA repair pathways make tumour cells highly sensitive to the inhibition of PARP activity....
Interferon-α directly inhibits DNA polymerase activity in isolated chromatin nucleoprotein complexes: correlation with IFN-α treatment outcome in patient... (1995) "Interferon-α directly inhibits DNA polymerase activity in isolated chromatin nucleoprotein complexes: correlation with IFN-α treatment ...
摘要: Compositions, methods, and kits for determining the activity of a nucleic acid polymerase are disclosed where fluorescent double stranded DNA binding dye and oligonucleotides are employed. Also disclosed are method for detecting inhibitory activity of a target molecule to a polymerase....
Poly(ADP-ribose) polymerase-1 (PARP-1) is a DNA-binding enzyme activated by DNA breaks and involved in DNA repair and other cellular processes. Poly(ADP-ribose) polymerase activity can be higher in cancer than in adjacent normal tissue, but cancer predisposition is reported to be greater in...