2. The diagram below represents a DNA nucleotide. Identify the phosphate group and deoxyribose. [1 mark] 3. Draw a labelled diagram to show how four nucleotides are joined together to form a double-stranded DNA molecule with two base pairs. [3 marks] 4. State two differences between RNA a...
The FAM-labelled probes (CA)16 and (CACG)8, marked as d and a, respectively, resulted in specific shift; they were competed out of the complexes by 100-fold excess of specific (Self) but not by the mutant (Mut) cold competitor. The BTB domain of ZBTB43 (ZBTB43-BTB) lacked binding...
No base skews were observed in the central regions of each molecule, arguing against a major influencer of Spo11-DC formation being localized DNA bending, which is expected to be favoured by an AT-rich base composition. i, The percentage of the total Spo11-oligo library in the filtered (...
Each TdT molecule is conjugated to a single deoxyribonucleoside triphosphate (dNTP) molecule that it can incorporate into a primer. After incorporation of the tethered dNTP, the 3′ end of the primer remains covalently bound to TdT and is inaccessible to other TdT–dNTP molecules. Cleaving the ...
Fig. 2.Mechanical properties of ss- and ds- DNA. (A) Diagram of a mechanical denaturalization experiment of dsDNA with optical tweezers. A single DNA molecule is tethered to functionalized polystyrene microspheres (beads) using biotin and digoxigenin moieties at the distal ends of one strand (blu...
In DNA origami, a nanostructure is assembled from a very long single-stranded scaffold molecule held in place by many short single-stranded staple oligonucleotides. Only the bacteriophage-derived scaffold molecules are amenable to scalable and efficient mass production23; the shorter staple strands are...
Signal transducing adapter molecule 2 TCF: T cell factor CLOCK: Clock circadian regulator MAPK: Mitogen-activated protein kinase MSTR1 : Macrophage-stimulating protein receptor GNG7 : Guanine nucleotide-binding protein subunit gamma-7 DAP-1: Disks large-associated protein 1 GKAP: Guanylate...
Since Z-DNA is a left-handed helix, its appearance in part of a DNA molecule helps to remove supercoiling stress. As negative supercoiling increases, the tendency for GC- or GT-rich tracts of DNA to take the Z-form increases. Its existence can be demonstrated in small plasmids by changes...
Nanopores are key in portable sequencing and research given their ability to transport elongated DNA or small bioactive molecules through narrow transmembrane channels. Transport of folded proteins could lead to similar scientific and technological benef
(100%, 101.36 a.u.). And the weak UCL signals of a, b and c samples may be nonspecific adsorption resulting from incomplete ExoIII reaction or the DNA molecule on the UCNPs surface. So, the proposed biosensor shows high specificity and high selectivity for target T1. In addition, the ...