2. The diagram below represents a DNA nucleotide. Identify the phosphate group and deoxyribose. [1 mark] 3. Draw a labelled diagram to show how four nucleotides are joined together to form a double-stranded DNA molecule with two base pairs. [3 marks] 4. State two differences between RNA a...
4d). As controls for the CC-containing constrained Z-DNA, we generated a flexible B-DNA circle by annealing a circular and a linear single-stranded DNA molecule (CL), and then ligating the second strand (Fig. 4d). We also generated B-DNA by annealing two linear DNA strands (LL). Z-...
Since Z-DNA is a left-handed helix, its appearance in part of a DNA molecule helps to remove supercoiling stress. As negative supercoiling increases, the tendency for GC- or GT-rich tracts of DNA to take the Z-form increases. Its existence can be demonstrated in small plasmids by changes...
The peak deflections segregated into two populations; we postulate that these may reflect the shape of the Qdot-protein complex bound to the DNA, thus providing a gross structural characterization of the molecule. This study is a demonstration of a technology that could be refined to tackle a ...
In DNA origami, a nanostructure is assembled from a very long single-stranded scaffold molecule held in place by many short single-stranded staple oligonucleotides. Only the bacteriophage-derived scaffold molecules are amenable to scalable and efficient mass production23; the shorter staple strands are...
As in the previous studies [1, 4–6], an amino acid residue was designated as a DNA or RNA-binding site if the side chain or backbone atoms of the residue fell within a cutoff distance of 3.5 angstroms (Å) from any atoms of the DNA or RNA molecule in the complex. All the other...
The 5′-FAM-labelled primer (10 pmol) was annealed to the template (15 pmol) in H2O by heating to 95 °C and then gradually cooling to room temperature (1 h). The appropriate polymerase, 5X or 10X enzyme buffer (provided by the supplier of the DNA polymerase), the natural nucleotides...
The 5 -end at each vertex of the polyhedron was labelled with biotin (green spheres at the bottom vertexes) to allow attachment to the streptavidin surface. (b) Process of structure assembly, target binding, and signal development for the APTEC assay. 2. Results 2.1. Design and Assembly of...
The present invention relates to a specific DNA sequence Tianma. 它们的长度:DNA序列1为360bp,DNA序列2为790bp;根据DNA序列的两端核苷酸序列设计的引物扩增各种天麻样本,以特异扩增条带鉴别各种天麻样本. Their length: DNA sequence 1 to 360bp, DNA sequence 2 is 790bp; designed according to the ...
the DNA molecule exhibited retarded migration on an agarose gel. Regardless of whether the sequence of the viral genome was involved in the DNA molecule, DNA retardation was not significantly influenced. This outcome indicated VP1 is a DNA binding protein with no sequence specificity. Various DNA ...