DNA MethylationEpigenetic epidemiologic studiesEpigeneticsEWASHuman diseasesEven though the importance of epigenetics was first recognized in light of its role in tissue development,an increasing amount of evidence has shown that it also plays an important role in the development and progression of many ...
In the review, we mainly summarize the studies exploring the role of aberrant DNA methylation as diagnostic and prognostic biomarkers in a broad range of human diseases, including monogenic epigenetics, autoimmunity, metabolic disorders, hematologic neoplasms, and solid tumors. The last section provides...
5.Lisanti, S., et al., Comparison of methods for quantification of global DNA methylation in human cells and tissues.PLoS One, 2013. 8(11): p. e79044. 6.Herman, J.G., et al., Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands.Proc Natl Acad Sci U...
[4] Stem Cell Research Unlocks a New Discovery about Controlling Genes. Retrieved Apr. 16, 2021 from https://www.mskcc.org/news/stem-cell-research-unlocks-new-discovery-about-controlling-genes [5] Li, W. et al., 2019. DNA Methylation Canyons in Human Cancers: Methods, Target Genes and F...
making it an ideal target for epigenetic studies in human populations. Here, using peripheral blood mononuclear cells collected from a community-based cohort stratified for early-life socioeconomic status, we measured DNA methylation in the promoter regions of more than 14,000 human genes. Using thi...
HumanMethylation450 BeadChip 当然,目前使用最多的芯片还是 450K 芯片;450K 芯片覆盖整个基因组的多个位置,而且相对比较便宜;可同时检测 12 个样本;每个样本覆盖 45 万个甲基化位点,而且可以做到碱基分辨率;而且适用于 FFPE 样本。 基于高通量测序的全基因组 DNA 甲基化检测方法 ...
美国纽约斯隆凯特琳研究所(Sloan Kettering Institute)的华人发育生物学家皇甫丹薇博士领导的团队对DNA甲基化(DNA methylation)展开了深入研究。DNA甲基化可以影响哪些基因最终产生蛋白质,是表现遗传的主要机制之一。 近日,皇甫丹薇博士与她的研究团队在《科学》杂志上发表论文,通过CRISPR筛选,鉴定出调节甲基化的一个关键...
近日,为了克服这些限制并准确表征人类细胞甲基化特征,来自以色列希伯来耶路撒冷大学等单位的研究团队在Nature发表了题为“A DNA methylation atlas of normal human cell types”的文章。研究团队构建了39种不同细胞类型的深度甲基化图谱,并将整...
et al. A systematic profile of DNA methylation in human cancer cell lines. Cancer Res. 63, 1114–1121 (2003). CAS PubMed Google Scholar Schlesinger, Y. et al. Polycomb-mediated methylation on Lys27 of histone H3 pre-marks genes for de novo methylation in cancer. Nat. Genet. 39, 232...
常见的甲基化芯片如Humanmethylation 450K,可以检测超过45万个CpG位点,覆盖99%已知的人类基因,包括启动子,5‘UTR,部分基因编码区,3’UTR等等。不同的芯片在检测方法的细节和检测范围上有一定的差异,可以根据需求选择。不过目前甲基化芯片类产品主要集中于人和小鼠样本的检测。