5Recipe6x Gel-loading Buffer I0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanol FF40% (w/v) sucrose in H2ODNA Size Migration with Sample Loading DyesAgarose concentration, %Xylene cyanol FFBromophenol blueOrange G0.7-1.74000bp300bp50bp2.5-3.0800bp100bp30bp6DNA在胶上的检测Ethidium ...
Table 6: Recipe for Buffer A.Prior to use, add half of a protease inhibitor tablet, 50 μL of 100 mM PMSF (1 mM final), 5 μL of 200 mM spermine (0.2 mM final), and 2.5 μL of 1 M spermidine (0.5 mM final) per 5 mL of solution. ...
•在做质粒的上有没有这个酶切位点鉴定的时候，用原质粒和经过单酶切的质粒在同一块胶上进行电泳，看单酶切后的质粒是不是比原质粒跑得快从而鉴定出质粒有没有被切开。Recipe6xGel-loadingBufferI0.25%(w/v)bromophenolblue0.25%(w/v)xylenecyanolFF40%(w/v)sucroseinH2O DNASizeMigrationwithSample...