6倍蔗糖DNA电泳上样缓冲液 CAS号: 英文名: 6 times sucrose DNA electrophoresis loading buffer 英文别名: 中文名: 6倍蔗糖DNA电泳上样缓冲液 中文别名: 6倍蔗糖DNA电泳上样缓冲液 CBNumber: CB45537192 分子式: 分子量: 0 MOL File: Mol file ...
After folding, monomer samples were analyzed by agarose gel electrophoresis (2% agarose, 0.5× TBE 10-mM MgCl2, 2-μL ethidium bromide) using Thermo Scientific EasyCast Mini Gel System. Twenty microliter of each sample was added to 4 μl of 6× loading buffer (60% glycerol, 1× TE, 0....
(Sangon). All the obtained proteins were dissolved in 5× Protein Loading Dye (Sangon) and then run on 8% SDS-PAGE gel in 1×Tris-Glycine running buffer using Mini-PROTEAN tetra system (BIO-RAD). The samples were run at 120 V for 1.5 h. The polyacrylamide gel was stained in ...
DNA is soluble in low-ionic-strength solution such as TE buffer or nuclease-free water. When such an aqueous buffer is applied to a silica membrane, the DNA is released from the silica, and the eluate is collected. The purified, high-quality DNA is then ready to use in a wide variety...
and then 6× gel-loading buffer, 0.25% (w/v)bromphenol blue(Sigma), 0.25% (w/v)xylene cyanolFF (Sigma), and 40% (w/v)sucrosein water are added to each sample to give a 1× gel-loading buffer. A 1% (w/v)agarosegel [1 g of agarose in 100 ml of 0.5× TBE (0.045MTris-bo...
The immunoprecipitants were washed six times with M2 buffer and boiled in 1 × SDS-loading buffer for immunoblot analysis. To detect cGAS acetylation in vivo, 3-week old Trex1–/– mice were given daily treatment of aspirin (50 mg/kg), salicylic acid (50 mg/kg), diclofenac sodium (1 ...
6紫外线照射不要太久 上样 每组加3个样品孔,记住位置 第一孔:9µl质粒DNA与1µl 6×loading buffer混匀后上样 第二孔:9µl酶切产物与1µl 6×loading buffer混匀后上样 第三孔:10µl PCR产物直接上样 四、结果与讨论:①结果:实验数据、现象、图谱;②讨论:以结果为根底的逻辑推论,并得出结...
CT26 cells were inoculated into a 6-well plate and allowed to proliferate for 12 h. Subsequently, (HAP NPs, HA, CH or CHA were added. After 24 h, cells were harvested and thoroughly washed with PBS. Cell pellets were added into RIPA lysis buffer (provided by Shanghai Biyuntian Biotechnol...
point samples were treated simultaneously with the RNeasy MinElute Clean-up kit (Qiagen) following the manufacturer’s protocol, with a final elution step in 14 µL milliQ. The samples were loaded with a 6× DNA loading buffer (Promega) on a 1.1% agarose gel containing EtBr and were ...
103Citations 16Altmetric Metrics Abstract Data storage in DNA is a rapidly evolving technology that could be a transformative solution for the rising energy, materials, and space needs of modern information storage. Given that the information medium is DNA itself, its stability under different storage...