线性DNA片段的环化离不开DNA连接酶（DNA ligase），真核生物共有三个DNA连接酶基因：Lig1，Lig3和Lig4。通过建立DNA连接酶的单基因敲除和多基因敲除细胞系。研究人员发现，Lig1和Lig4的缺失完全不妨碍eccDNA的生成，而Lig3缺失的细胞系中的eccDNA产量则急剧降低，说明Lig3是最主要的负责DNA环化的连接酶（图2）。
线性DNA片段的环化离不开DNA连接酶 (DNA ligase) ,真核生物共有三个DNA连接酶基因:Lig1,Lig3和Lig4。通过建立DNA连接酶的单基因敲除和多基因敲除细胞系。研究人员发现,Lig1和Lig4的缺失完全不妨碍eccDNA的生成,而Lig3缺失的细胞系中的eccDNA产量则急剧降低,说明Lig3是最主要的负责DNA环化的连接酶 (图2) 。
线性DNA片段的环化离不开DNA连接酶(DNA ligase),真核生物共有三个DNA连接酶基因:Lig1,Lig3和Lig4。通过建立DNA连接酶的单基因敲除和多基因敲除细胞系。研究人员发现,Lig1和Lig4的缺失完全不妨碍eccDNA的生成,而Lig3缺失的细胞系中的eccDNA产量则急剧降低,说明Lig3是最主要的负责DNA环化的连接酶(图2)。这回答...
DNA ligase III isoforms α and β include an N-terminal zinc finger (ZnF; pink). DNA ligases IIIα and IV have a breast and ovarian cancer susceptibility protein 1 (BRCT) C-terminal domain (red). While DNA ligases I and IIIβ have a nuclear localization signal (NLS; gray), the N-...
18.Caldecott KW, Aoufouchi S, Johnson P, Shall S. 1996. XRCC1 Polypeptide Interacts with DNA Polymerase and Possibly Poly (ADP-Ribose) Polymerase, and DNA Ligase III Is a Novel Molecular 'Nick-Sensor' In Vitro. Nucleic Acids Research. 24(22):4387-4394.19.Dantzer F, de la Rubia G,...
DNA ligase 1 finalizes nuclear DNA replication by accurately sealing nicks generated during Okazaki Fragment Maturation. Here, Williams et al employ a low fidelity DNA ligase 1 variant to study mutation rates and specificity across the yeast genome ...
The three steps of a DNA ligation reaction (Figure 1): The DNA ligase is self-adenylated by reacting with ATP or NAD+, creating a phosphamide bond between a lysine residue within the enzyme and an AMP molecule of the cofactor, either ATP or NAD+. The adenyl group is transferred to 5...
The anaphase-promoting complex/cyclosome (APC/C) is a large multiprotein E3 ubiquitin ligase involved in ubiquitin-dependent proteolysis of key cell cycle ... NB Eloy,G Nathalie,VL Jelle,... 被引量: 0发表: 2018年 Transcription factor AtDOF4;2 affects phenylpropanoid metabolism in Arabidopsis ...
A. DNA ligase required for active DNA demethylation and genomic imprinting in Arabidopsis. Cell Res. 25, 757–760 (2015). Article PubMed Central CAS PubMed Google Scholar Ponferrada-Marin, M. I., Roldan-Arjona, T. & Ariza, R. R. Demethylation initiated by ROS1 glycosylase involves ...
DNA酶Ⅰ样蛋白3(DNASE1L3)重组蛋白 纯度: Greater than 85% as determined by SDS-PAGE. 基因名: DNASE1L3 Uniprot No.: Q13609 别名: Deoxyribonuclease gamma; Deoxyribonuclease I like 3; Deoxyribonuclease I like III; Deoxyribonuclease I-like 3; DHP 2; DHP2; DNAS1L3; DNase gamma; DNase I homol...