Use VectorBuilder's free DNA reverse complement tool to transform any DNA sequence of your choice into its reverse, complement or reverse-complement.
PCR bias is not correlated with GC content Although previous studies observed PCR bias in genomic biological sample amplification15,16,17, it remained unclear whether such bias is significant in DNA data storage. To assess this, we used the 1.5 million-sequence ready-to-sequence pool and compared...
For indexing PCR, the following was added to each well of the ligation plate: 10 µL 5X VeraSeq GC Buffer, 2 µL 10 µM dNTPs, 1.5 µL VeraSeq Ultra Enzyme, 24 µL dH2O, 0.5 µL 100X EvaGreen, 1 µL 10 µM TruSeq i5 primers, 1 µL 10...
Purified cytosolic DNA was tested for nuclear DNA contamination by performing a 40-cycle PCR reaction using primers for vGlut1 (also known as Slc17a7) (forward: GTGGAAGTCCTGGAAACTGC, reverse: ATGAGCGAGGAGAATGTGG). For cloning of dsDNA, samples were treated with DNA polymerase I, large (...
CODEX2 by default is for hg19 reference. It can be adapted to hg38: only the calculations of GC content and mappability need to be changed; to get coverage for exons across samples stays the same (make sure that the exonic targets in the bed file are also in hg38 coordinates). To cal...
(equivalent to 32 16, 8, and 4 Adj. CpG Layers respectively) for X-chromosome.CChrX:71.4–71.6 Mb. GVIZ track stack plot. Top track shows mean β value per group, next track shows Δβ, where Δβ = βfemale−βmale. Below the gene model track is the DMR track, highlighting...
Other applications might include identification of sequences from their quasi-alignment models and finding interesting regions within sequences, such as those with high GC content. In this paper we have restricted our discussion to creating non-overlapping segments. For dealing with sequences which ...
More interestingly, the level of SAMD12-AS1 was found to be correlated with GC patient tumor grade and prognosis. The transcription factor p53 is a tumor suppressor, which activates downstream targets to induce cell cycle arrest and trigger apoptosis (24). Interestingly, studies have also reported...
Using a bioinformatics approach, we identified large TR with array size more than 3 kb in two mouse whole genome shotgun (WGS) assemblies. Large TR were classified based on sequence similarity, chromosome position, monomer length, array variability, and GC content; we identified four superfamilies...
With the Cooper-Helmstetter model, one can derive origin number (nori) and genome content (Gc) as a function of C, D, and τ: nori = 2(C + D)/τ (90) and Gc = [τ/(C× ln2)][2(C + D)/τ − 2D/τ] (7). We used these two theoretical formulas to check whether a ...