You can therefore choose an optimal biotinylation reagent specific for your application without inactivating your target molecule. The high stability and binding capacity of the DNA on Dynabeads allows binding of the target proteins with kinetics similar to that of DNA in free solution...
Biotinylation of oligonucleotides Immobilize the biotin-labeled DNA/RNA target sequence onto the beads and incubate with the cell extract. You can then isolate the bound proteins using magnetic separation. You can elute off the protein for characterization or apply the whole soli...
There are a variety of commercially available biotinylation reagents that target different functional groups like primary amines, sulfhydryls, carboxyls, carbohydrates, tyrosine and histidine side chains, and guanidine and cytosine bases. All biotin reagents should contain a spacer ...
KAS-seq是一种基于叠氮基-乙氧二羟丁酮(N3-kethoxal)和鸟嘌呤在单链DNA中的快速和特异性反应的单链DNA高通量测序方法(图3)。N3-kethoxal能对单链DNA上的鸟嘌呤进行特异性标记,它可以在37 ℃下5 min内特异性地与活细胞中单链状态DNA中的鸟嘌呤...
The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3'-OH end of single-stranded DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt ...
The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3'-OH end of single-stranded DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt ...
电话:020-29031124 手机:13760848131 Email:servers@gzscbio.com Fax:020-29031124 QQ:2913120624 您当前所在的位置:首页>>产品展示>>生物试剂>>专题试剂>>EMSA实验试剂 DNA 3' End Biotinylation Kit 产品名称:DNA 3' End Biotinylation Kit 品牌名称:Thermo SCIENTIFIC ...
The biotinylation/PCR products are then exposed to a bearing, will selectively bond to biotinylated stock to allow the product of selective elution.MITCHELL LLOYD G.MERRIL CARL R.
O’Sullivan和他的团队利用最近开发的一种被称作邻近依赖性生物素化(proximity dependent biotinylation, BioID)的技术解决了这个问题,其中这个技术允许他们快速地鉴定出物理上接近的因而与癌细胞中的端粒延长潜在相关联的蛋白。 当对端粒酶或ALT通路有活性的癌细胞进行比较时,这种BioID技术鉴定出139种ALT通路有活性的癌...
High-molecular-weight DNA was removed by reverse size selection with 1.2× PCR clean up beads (MagBio), and samples were subjected to biotinylation with biotin-16-ddUTP (Merck). Samples were separated on 12% urea–polyacrylamide gel, transferred to hybond N+ nylon membrane (Amersham) and ...