Biotinylation of oligonucleotides Immobilize the biotin-labeled DNA/RNA target sequence onto the beads and incubate with the cell extract. You can then isolate the bound proteins using magnetic separation. You can elute off the protein for characterization or apply the whole solid-pha...
Biotinylation of oligonucleotides Immobilize the biotin-labeled DNA/RNA target sequence onto the beads and incubate with the cell extract. You can then isolate the bound proteins using magnetic separation. You can elute off the protein for characterization or apply the whole soli...
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Sequencing gel analysis confirms efficient biotinylation of oligonucleotides Ten different oligos (ranging in size from 21-25 nt) were labeled using the Thermo Scientific Biotin 3' End DNA Labeling Kit. The products from the TdT reaction were then radiolabeled using T4 polynucleotide kinase (PNK) and...
The Thermo Scientific Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods. The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase...
BiotinylationUnneeded ATP biotinylation processThis study describes the development of cryogel columns prepared by ATP having ruthenium hapten cross-linker based photosensitive approach \"ANADOLUCA\" for the biotinylation of Crimean Congo Hemorrhagic Fever (CCHF) primers through 5' end as a first. In ...
available (InvivoGen) and can be readily visualized after transfection with no additional labeling. An alternative approach isbiotinylationof unlabeled DNA (plasmid or other), followed byfluorescent labelingwith Streptavidin-coupled fluorophores. The PHOTOPROBEbiotinkit (Vector Labs) serves well for this...
For RNA pulldown experiments, RNA of HIF1α-AS1 or of the control pcDNA3.1+ were further biotinylated at the 3ʹend with the Pierce RNA 3ʹend biotinylation kit (Thermo Fisher). RNA pulldown assay and mass spectrometry The RNA pulldown assay was performed similar to39. For proper RNA...
High-molecular-weight DNA was removed by reverse size selection with 1.2× PCR clean up beads (MagBio), and samples were subjected to biotinylation with biotin-16-ddUTP (Merck). Samples were separated on 12% urea–polyacrylamide gel, transferred to hybond N+ nylon membrane (Amersham) and ...
Biotinylation of the 5' end of these reference DNAs was achieved by conjugating biotin to the appropriate primer for PCR, which in turn allowed these molecules to serve as capture DNA after binding to streptavidin-coated plates. Up to 27 reference HIV- DNAs (Table 2) and 6 reference HCV ...