This information is particularly useful within the context of restoration ecology. In this study, DNA barcoding is used to assess the potential of Hemiptera as a biological indicator of restoration success for the Buffelsdraai Landfill Site Community Reforestation Project (Durban, South Africa). A ...
05 October 2015 Published: 30 October 2015 A DNA Mini-Barcoding System for Authentication of Processed Fish Products Shadi Shokralla1,2, Rosalee S. Hellberg3, Sara M. Handy4, Ian King1 & Mehrdad Hajibabaei1 Species substitution is a form of seafood fraud for the purpose of...
Here, we provide a thorough and updated vision on the recentlyreported approaches that are applying these DNA-based tools to assessthe authenticity of food components and products. 展开 关键词: PCR DNA barcoding next-generation sequencing foodomics food fraud food authentication DNA ...
The development of droplet-based encapsulation and barcoding has enabled the analysis of thousands or even millions of single cells and the genome-wide analysis of open chromatin and gene expression changes. Initial analysis of a limited number of samples has allowed for the identification of cell-...
DNA barcoding was used for seafood species identification, and the scientific names retrieved from the sequence analysis after consulting BOLD (Barcode of Life Data System) and GenBank were compared with the expected species, genus, and family to determine the description authenticity (Fig. 2). ...
DNA barcoding is a technique for identifying species by analyzing short DNA regions, such as genetic markers. The length of the DNA barcode marker should be long enough to obtain sufficient taxonomic information, but short enough to meet the constraints of sequencing resources. For instance, the ...
Furthermore, innovations have surged in the domain of target enrichment strategies, alongside the integration of multiplexing and barcoding approaches. These physical advancements are mirrored in the digital realm, with bioinformatics pipelines now encompassing sophisticated variant calling algorithms, stringent...
[47], which we demonstrate here for 16 species-identified elasmobranchs. The shark-specific COI primers used here amplify a 127-bp region of a widely-used barcoding gene that has large reference databases (e.g. GenBank, BOLD) for sequence matching [65,67,68] and for which there is ...
Here, we tested how a DNA barcoding approach based on high-throughput sequencing of sorted and genetically tagged specimens performed to assess oligochaete species diversity and abundance and the biological quality of sediments in streams and lakes. We applied both molecular and morphological approaches ...
DNA barcoding is currently an effective and widely used tool that enables rapid and accurate identification of plant species. The Orchidaceae is the second largest family of flowering plants, with more than 700 genera and 20,000 species distributed nearl