DNA barcoding is commonly used for species identification. Despite this, there has not been a comprehensive assessment of the utility of DNA barcoding in crayfishes (Decapoda: Astacidea). Here we examined the extent to which local barcoding gaps (used for species identification) and global ...
MT-COI is widely used for DNA barcoding as it has both conserved regions and segments with high divergence (Hebert et al. 2003). Recent work identified a segment of MT-COI capable of discerning wild and domestic goat and sheep taxa in Central Asia, making it an important addition for diet...
This technique enables the encapsulation of genomic DNA within intact nuclei during an initial round of cell barcoding. Subsequent processing, including bisulfite conversion, adapter tagging, and PCR amplification is then carried out on pools of cells; greatly reducing costs and enabling high throughput...
MT-COI is widely used for DNA barcoding as it has both conserved regions and segments with high divergence (Hebert et al. 2003). Recent work identified a segment of MT-COI capable of discerning wild and domestic goat and sheep taxa in Central Asia, making it an important addition for diet...
The validity of DNA barcoding relies heavily on the availability of a precise reference database, as it serves as the cornerstone of DNA barcoding. In this study, we constructed a comprehensive ITS2 matrix encompassing genuine NReR and their commonly found adulterants for the first time. This ma...
Short fordeoxyribonucleic acid.The nucleic acid that is the genetic material determining the makeup of all living cells and many viruses. It consists of two strands of nucleotides linked together in a structure resembling a ladder twisted into a spiral. In eukaryotic cells, the DNA is contained ...
DNAbarcoding(条形码)isthederivationofshortDNAsequence(s)thatenablesspeciesidentification,recognition,anddiscoveryinaparticulardomainoflife.2 Whatisbarcoding?DNAbarcodingwillcomplement—notsupplantorinvalidate—existingtaxonomic（分类学）practices；ThegoalsofDNAbarcodingisdifferentfromDNAtaxonomy；Ideally,...
Sanger dideoxy sequencing is vital in clinical analysis due to its accuracy, ability to analyze genetic markers like SNPs and STRs, capability to generate reliable DNA profiles, and its role in resolving complex clinical cases. The precision and robustne
Mayflies, stoneflies and caddisflies (Ephemeroptera, Plecoptera and Trichoptera) are prominent representatives of aquatic macroinvertebrates, commonly used as indicator organisms for water quality and ecosystem assessments. However, unambiguous morphological identification of EPT species, especially their immat...
The success of parasitoids is in turn a clue to the previously unexplored diversity of their hosts, especially other Holometabola. Full species delimitation and description require more detail than DNA barcoding alone can provide; these are questions best answered by integrative methods that ...