通常在细胞间期包括复制期，三维基因组的数据（HiC，ChIA-PET，HiChIP 等）以二维互作图的形式展示，其最具特征性的表征是相对于二维对角线45度角所形成的染色质环（chromatin loop）和结构域 (TAD)，但是极少能直接观察到类似于由 Repli-HiC 所发现的垂直于二维对角线的染色质喷泉（chromatin fountain）。这一发现...
The DNA damage response (DDR) is a signaling cascade that is triggered by DNA damage, involving the halting of cell cycle progression and repair. It is a key event leading to senescence, which is characterized by irreversible cell cycle arrest and the se
In subject area: Agricultural and Biological Sciences The term non-B-DNA refers to all secondary structures that the canonical, antiparallel right-handed double helix (B-DNA) can adopt, including left-handed duplexes and three- and four-stranded helices. From: Encyclopedia of Biological Chemistry ...
The methylated sites, especially the promotor regions, influence the protein-DNA interactions leading to chromatin remodeling and affecting the expression of specific genes [6,9]. Many chemical agents (5-azacytidine, 6-thioguanine, 5-(β-d-Ribofuranosyl) isocytosine, and dexamethasone cytarabine ...
In neurons, HSV may enter a latent phase wherein the virus persists as a benign episomal entity in the cell nucleus. Genes may be inserted to replace the latent viral genes and can then be expressed for the lifetime of the cell. Although HSV is also a sizable virus, the HSV genomic ...
b, Exemplar DNA and chromatin profiles of AIRE-induced genes poised for expression in mTECs from Aire-KO mice. In comparison, exemplar profiles for an ANG were shown on the right. c, Same as Fig. 4d except WT ATAC-seq and ChIP-seq signals. p-values in panels a and c were calculated...
DNA replication initiates from replication origins firing throughout S phase. Debate remains about whether origins are a fixed set of loci, or a loose agglomeration of potential sites used stochastically in individual cells, and about how consistent thei
in more sensitivity to Vibrio cholera infections in mice [21]. Due to lack of an aspartate in the catalytic center, ARH2 appears to be catalytically inactive [5]. ARH3 plays important roles in neurodegenerative disease [22] and efficiently uses PAR chains, MARylated serine and OAADPr as a...
(transcription start sites) and appears to mark active genes [18,21]. In rice, 6mA is depleted from the TSS and is associated with no expression when it marks in the promoter but is associated with gene transcription when it marks in gene bodies [16]. InArabidopsis, 6mA is widespread ...
After nuclear transfer to eggs, sperm and transplanted somatic nuclei undergo a substantial enlargement and decondensation of chromatin. As soon as 20 minutes after fertilization at 18°C, DNA synthesis is initiated in the sperm nuclei. This first round of DNA synthesis is completed in the next ...