During interphase the chromosomes cannot be identified individually, since they are 'unrolled'. When mitosis begins the chromosomes become visible as chromatids arranged in pairs (see section 1.4.2). In this phase, morphological differentiation of the chromosomes is possible. There are two different...
pro·phase (prō′fāz′) n. 1.The first stage of mitosis, during which the chromosomes condense and become visible, the nuclear membrane breaks down, and the spindle apparatus forms at opposite poles of the cell. 2.The first stage of meiosis, constituted by a series of events that include...
Overall, these results suggest that most developmentally regulated genes do not develop tissue-specific chromatin organization over this developmental period, further arguing that tissue-specific chromatin organization is not required for tissue-specific expression during this developmental transition. Micro-C ...
Little is known about how the epigenomic states change during development and evolution in a 3D genome context. Here we useDrosophila pseudoobscurawith complex turnover of sex chromosomes as a model to address this, by collecting massive epigenomic and Hi-C data from five developmental stages and ...
Fasting is a major environmental cue across the animal kingdom, yet how it impacts three-dimensional (3D) genome organization is unknown. Here we show that fasting induces an intestine-specific, reversible and large-scale spatial reorganization of chromatin in Caenorhabditis elegans. This fasting-...
We found that Fkbp39 can phase separate with nucleosomes and form large liquid-like condensates (Figure 1D). By contrast, Fkbp39 only weakly phase separates with DNA, forming very small condensates at concentrations where homotypic FKBP39 phase separation is favored (Figures S2D–S2F). This ...
While the conventional txci-ATAC-seq method enables sample multiplexing, its efficiency is hindered by the labor-intensive nature of nuclei washing and counting procedures, constraining the number of samples (typically no more than 12) that can be processed in a single day. To address this limita...
Using our established cell cycle synchronisation system by serum deprivation, we showed with the aid of qRT-PCR that Par14 and Par17 were 2- and 3-fold up-regulated respectively during the S-phase at the mRNA level. A 3- and 5-fold up-regulation was seen for Par14 and Par17 in the...
To test whether the formation of an immiscible chromatin phase is suppressed by acetylation, we treated cells with TSA before mitotic entry and then injected AluI. This resulted in homogeneously dispersed chromatin fragments with almost no local condensates (Fig.2e,fand Supplementary Video5; the few...
20) and can self-oligomerize and recruit H3K9 methyltransferases potentially contributing to heterochromatin compaction21,22, spread23,24 and phase separation25,26,27. DNA methylation is associated with both heterochromatin and extrusion barriers. In humans, the DNA methyltransferase DNMT1 physically ...