In order to do so, we took advantage of the Clover tag in TRAIP-mAC and looked for a proximity signal arising from TRAIP-mAC and RNA Pol II in the cells, using GFP antibody to detect TRAIP-mAC using the PLA technique. We could indeed detect such a proximity that was lost upon auxin...
To test whether SYCP2 levels are relevant to the DDR drug response in patients, we analyzed 16 breast cancer patients (7 luminal ER/PR positive and 8 triple-negative with no known BRCA mutations, and 1 unknown BRCA status) treated with Sacituzumab Govitecan (IMMU-132), an antibody-conjuga...
kinase of DNA-PKcs, whole cell lysates of Jurkat T cells that transfected with ISD or 293T cells that transfected with Flag-Ku70 were pre-cleaned with Protein-A/G-coupled agarose beads, and then immunoprecipitated with the anti-KU70 or anti-Flag antibody on a shaker at 4°C for over...
We also examined Cas9- or nCas9-induced focus formation of γH2AX and 53BP1, both of which serve as a DSB marker. Both Cas9 and nCas9-induced co-localized formation of γH2AX and 53BP1 foci as did bleomycin, HU and CPT (Fig.1cand Supplementary Fig.1b, c). Cas9Dinduced even more...
Catecholamine-mediated STAT3 activation was not inhibited by pretreatment with an anti–interleukin 6 (IL-6) antibody or with small interfering RNA (siRNA)–mediated decrease in IL-6 or gp130. Regarding the effects of STAT3 activation, exposure to norepinephrine resulted in an increase in invasion...
FIG. 1: Overview of variations of the antibody toxin scFv(FRP5)-ETA and specific sequence characteristics (A) Constructs for bacterial expression of scFv(FRP5)-ETA derivatives. All expression cassettes are under the control of the IPTG-inducible tac-promoter and code for the ErbB2-specific scFv...
JCV early proteins were immunoprecipitated with the monoclonal antibody PAb 962, separated on an 18% SDS- polyacrylamide gel and transferred to nitrocellulose membranes. Western blot analysis was performed using PAb 2003 as the primary antibody and a goat ®-mouse HRP and un(der)phosphorylated T16...
Blots were reprobed with anti-HSP90 antibody to confirm equal loading. d B2M gene alterations on 64 CRC cell lines at T0 (upper panel) and codon affected (lower panel) Our analysis suggests that in parallel to mutation gains, loss of variants also occurs during cell propagation. Our data ...
c Western blot indicates the specificity of the C11orf54 antibody in the C11orf54 knockdown cells and reintroducing C11orf54 in the C11orf54 knockdown cells. d Representative immunostaining images confirm the subcellular localization of C11orf54 in wild-type cells. Scale bar = 20 μm. e...
Supplementary Table S1. Antibody used in this work; Table S2: Supplementary Table S2. Sequences of all primers and probes used in this work; Table S3: In vitro IC50concentrations for CX-5461 and CPT; Supplementary Data S1: Sequence of pcfTAF110 plasmid; Supplementary Data S2: Sequence of ...